نتایج جستجو برای: Single-Strand Break (SSB)
تعداد نتایج: 928853 فیلتر نتایج به سال:
introduction the aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (appj) and interactions of this technology with double-stranded dna using the polymerase chain reaction (pcr) and single-strand conformation polymorphism (sscp) techniques. materials and methods the plasma jet was produced through a high voltage sinusoidal power supplyusing a mixt...
Mating-type switching in the fission yeast Schizosaccharomyces pombe is initiated by a strand-specific imprint located at the mating-type (mat1) locus. We show that the imprint corresponds to a single-strand DNA break (SSB), which is site- but not sequence-specific. We identified three novel cis-acting elements, involved in the formation and stability of the SSB. One of these elements is essent...
Introduction: Passage of ionizing radiation through the organs of living creatures develops clusters of damaged nucleotides inside the DNA rounds. 192Ir Gamma source is one of the most widely used sources in brachytherapy of cervical and prostate cancer. Thus, in this research, we investigated the flux of photons and its resulting secondary electrons, the single-strand break (S...
Radiotherapy using various beams is one of the methods for treating cancer, Hadrons used to treat cancers that are near critical organs. The most important part of the cell that is damage by ionizing radiation is DNA. In this study, damages induced in the genetic material of living cells (DNA) defined by the atomic model from the protein data bank (PDB) have been studied by radiati...
The Deinococcus radiodurans SSB protein has an occluded site size of 50 +/- 2 nucleotides on ssDNA but can form a stable complex with a 26-30-nucleotide oligodeoxynucleotide using a subset of its four ssDNA binding domains. Quantitative estimates of D. radiodurans SSB protein in the D. radiodurans cell indicate approximately 2500-3000 dimers/cell, independent of the level of irradiation. At bio...
Embryonic stem cells (ESCs) enable rapid proliferation that also causes DNA damage. To maintain genomic stabilization during rapid proliferation, ESCs must have an efficient system to repress genotoxic stress. Here, we show that withdrawal of leukemia inhibitory factor (LIF), which maintains the self-renewal capability of mouse ESCs (mESCs), significantly inhibits the cell proliferation and DNA...
As the most common type of DNA damage, DNA single-strand breaks (SSBs) are primarily repaired by the SSB repair mechanism. If not repaired properly or promptly, unrepaired SSBs lead to genome stability and have been implicated in cancer and neurodegenerative diseases. However, it remains unknown how unrepaired SSBs are recognized by DNA damage response (DDR) pathway, largely because of the lack...
The human SSB homologue 1 (hSSB1) has been shown to facilitate homologous recombination and double-strand break signalling in human cells. Here, we compare the DNA-binding properties of the SOSS1 complex, containing SSB1, with Replication Protein A (RPA), the primary single-strand DNA (ssDNA) binding complex in eukaryotes. Ensemble and single-molecule approaches show that SOSS1 binds ssDNA with...
The DNA damage response (DDR) plays a pivotal role in maintaining genome integrity. DNA damage and DDR activation are observed in the failing heart, however, the type of DNA damage and its role in the pathogenesis of heart failure remain elusive. Here we show the critical role of DNA single-strand break (SSB) in the pathogenesis of pressure overload-induced heart failure. Accumulation of unrepa...
The mechanism of DNA strand break induction due to K-shell photoabsorption of DNA constituent phosphorus was studied by computer simulation. Photon induced Auger process in DNA solution was modeled and introduced to the Monte Carlo simulation code DBREAK, which allows the estimation of DNA strand break production on pBR322 plasmid DNA in aqueous solution. The simulation was carried out for the ...
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