abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...

the sigatoka disease complex of banana involves three related ascomycetous fungi viz., mycosphaerella fijiensis, m. musicola, and m. eumusae. due to rather similar symptoms on banana, similar teleomorph morphology and problems associated with growth and sporulation in culture, species identification based on classical methods is troublesome. the exact distribution of these three species and the...

objective: utility of pcr-rflp and species-specific pcr as novel and fast methods for identification and discrimination of causative agents of relapsing fever, borrelia persica and b. microtii in infected blood were investigated. materials and methods: genomic dna of b.persica and b.microtii species were extracted from the highly infected blood samples. two fragments of glpq and 16srdna genes ...

Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

an experiment was conducted, using two year old kinnow plants, budded on rough lemon rootstock, inoculated with different isolates of amf fungi viz., glomus manihotis, gigaspora gigantean and glomus mosseae singly as well as in mixed culture. five primers vans1, ns61,ns21,vaglo,vagiga specific to glomus and gigaspora species were designed and used in a polymerase chain reaction with dna extract...