Initial studies to establish an in vitro system allowing survival and multiplication of porcine spermatogonia are described. Purified spermatogonia from 3-week-old pigs were cultured for 9 days alone or in the presence of Sertoli cells in either control medium or in medium supplemented with 5%, fetal calf serum (FCS). Under either condition the number and the viability of the cells decreased wi...

The present study examines the existence of clonogenic patterns in the proliferation and differentiation of spermatogonial stem cells in two species of non-human primates, the marmoset and the rhesus monkey. We developed a novel approach to detect proliferating spermatogonial clones in whole mounts of seminiferous tubules. Dual fluorescence labeling of bromodeoxyuridine and acrosin in conjuncti...

A procedure is described which permits the isolation from the prepuberal mouse testis of highly purified populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes, leptotene and zygotene primary spermatocytes, pachytene primary spermatocytes and Sertoli cells. The successful isolation of these prepuberal cell types was accompli...

The spermatogenesis and oogenesis-specific transcription factor Sohlh2 is normally expressed only in premeiotic germ cells. In this study, Sohlh2 and several other germ cell transcripts were found to be induced in mouse embryonic stem cells when cultured on a feeder cell line that overexpresses bone morphogenetic protein 4. To study the function of Sohlh2 in germ cells, we generated mice harbor...

diazinon is an organophosphate which exerts tissue damages by generating free radicals thereby inducing oxidative stress. the purpose of this study is to determine the protective effect of salvia officinalis l.extract on testes of rats intoxicated by diazinon. in this experimental study, 45 mature male rats were equally divided into three groups of control (c), diazinon (dzn) and diazinon-extra...

AIM To investigate whether estrogen stimulates the proliferation of spermatogonia or induces spermatogenesis in cryptorchid mice. METHODS Mice were surgically rendered cryptorchid, then treated with different doses of 17beta-estradiol (E2) s.c. once a day. Mice were killed at sexual maturity (45 days of age), and histological analysis and immunofluorescence were performed. Serum follicle stim...

Studies on circaannual changes in reproduction of Rainbow trout (Oncorhynchus mykiss), an economically important fish of Kashmir have been carried out. The variations in testicular structure and function of rainbow trout were studied during the present investigation. Morphometric and gravimetric data revealed four distinct reproductive phases viz. (I) Resting or Spent stage (II) Maturation stag...

Spermatogenesis is a continuous and highly coordinated process of spermatozoa production. In mice, this process is believed to initiate shortly after birth with the emergence of nascent spermatogonia in the testes. However, because the nascent spermatogonia originated from the gonocytes are morphologically indistinguishable from their predecessors and there is no clear definition for the gonocy...

This article contains the data related to the research article "in vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay" (Uchida et al., 2016) [1]. A novel transplantation assay of growth factor-soaked beads into the mammalian testicular interstitium was developed, in order to examine the effects of various soluble factors on in vivo dynami...

Vitamin A is instrumental to mammalian reproduction. Its metabolite, retinoic acid (RA), acts in a hormone-like manner through binding to and activating three nuclear receptor isotypes, RA receptor (RAR)α (RARA), RARβ, and RARγ (RARG). Here, we show that 1) RARG is expressed by A aligned (A(al)) spermatogonia, as well as during the transition from A(al) to A(1) spermatogonia, which is known to ...