introduction: nowadays, spermatogonial stem cells (sscs) cultivation has been used by many researchers as an effective tool for infertility treatments. oxidative conditions can be effective on cell proliferation and differentiation of these cells. so, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during sscs in vitro culture. ...

background: in most mammals, the testis is always maintained at a lower temperature than that in the abdomen, and exposure of the testis to body temperature causes degeneration of germ cells. objective: in this research, the long effect of heat exposure on sperm parameters and microstructure of mouse testis were investigated. cryptorchid mouse were induced by exposure to abdominal heat. materia...

stem cells are generally defined as clonogenic cells capable of both self-renewal and differentiation. probably the best method for long-term preservation of spermatogonial stem cells is cryopreservation. in this study, effects of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing were investigated. sertoli and spermatogonial ...

Background: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. To investigate and biomanipulate these cells, first the proliferation and viability rate of cells s...

objective: it is hypothesized that stem cells have the capability to form tumors after transplantation. spermatogonial stem cells have proliferation potency and colonization ability related to express pluripotency genes such as c-myc. the primary aim of this study is to investigate tumorigenicity ability of these cells after in vitro cultivation and inoculation in athymic animals. methods: sper...

objective: the properties of self-renewal and divisionin spermatogonial stem cells (sscs) support spermatogenesis. there are a number of reported methods for in vitro ssc culture systems. the development of a culture system that effectively supports isolation and self-renewal ofgermline stem cells (gscs) is of tremendous benefit for clinical trials, experimental research andas potential treatme...

background:   objective: this study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis   materials and methods: isolated testicular cells were cultured in dmem/f12. characteristics of the isolated cells and obtained es-like cell were immune-cytochemically confirmed by examining the presence of plzf, vimentin, oct4 and nanog prote...

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

background: the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. to investigate and biomanipulate these cells, first the proliferation and viability rate of cells s...

the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells (sccs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. to investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...