in order to study the effect of pre and postharvest polyamines spraying on increasing vase life of alstroemeria (alstroemeriaaurantica cv.sukari), an experimental was conducted on completely randomized factorial design with two types polyamines (putrescine, spermine) in four levels (zero, five, 10, 15 and 20 mg/l). results showed that, 20 mg/l of putrescine and spermine treatments increased vas...

adenosine deaminase is an aminohydrolase (ec 3.5.4.4) which participates in the purine metabolism where it degrades either adenosine or 2'-deoxyadenosine producing inosine or 2'-deoxyinosine, respectively. the enzyme contains a parallel alpha/beta -barrel motif with eight central beta strands and eight peripheral alpha helices. ada is located both in the cytosol and on the cell membrane. since ...

this study was performed to investigate the effect of putrescine and spermine on some physiological and biochemical characteristics and antioxidant enzymes activity of peach (prunus persica cv. alberta) and almond (prunus dulcis cv. rabi) seedlings under water deficit. the first factor was water regime at two levels (80% or 50% of soil available water), and the second factor was polyamine treat...

Investigation was done to find out the effect of different polyamines viz., spermine and spermidine (5, 10, 20 mg/l) biostimulants 2 % Novel (banana pseudostem sap) 2% cow urine as foliar spray on growth parameters rose var. Top Secret.
 Plant height, number branches leaves were found be significantly higher with biostimulants. Maximum plant height in plants treated mg/l while maximum sper...

The new natural polyamine conjugate 1N,5N,10N,14N-tetracoumaroyl spermine (tetracoumaroyl spermine) recently isolated from chamomile (Matricaria chamomilla L.) flower heads is applicable for the treatment of several human disorders such as depression and anxiety. High variability in the level of tetracoumaroyl spermine is found in commercial tisanes. Accumulation of tetracoumaroyl spermine was ...

In this work, we have studied the transcriptional profiles of polyamine biosynthetic genes and analyzed polyamine metabolic fluxes during a gradual drought acclimation response in Arabidopsis thaliana and the resurrection plant Craterostigma plantagineum. The analysis of free putrescine, spermidine and spermine titers in Arabidopsis arginine decarboxylase (adc1-3, adc2-3), spermidine synthase (...

High local polyamine concentrations may suppress cell growth of primary prostatic carcinomas. When cell growth assays were conducted in defined serum-free medium, spermine inhibited the growth of poorly metastatic rat prostate carcinoma cells, causing cell cycle arrest in the G1 phase. In contrast, highly metastatic prostate carcinoma cells were resistant to the growth inhibitory activity of sp...

The potential polyamine antagonist action of N1-dansyl-spermine (a potent NMDA antagonist) was assessed in two in vivo mouse models of polyamine action. Co-administration of N1-dansyl-spermine (2-10 microg, i.c.v.) with spermine (100 microg, i.c.v.) resulted in a dose-dependent antagonism of the spermine-induced CNS excitation (body tremor and fatal tonic convulsions). In addition, the same dos...

Spermine is the end-product in the polyamine biosynthetic pathway, and its excess accumulation induces neuroexcitatory responses and neurotoxicity. The purpose of this study was to elucidate the involvement of transport systems at the brain barriers in the clearance of spermine. In vivo rat spermine elimination from brain parenchyma across the blood-brain barrier (BBB) and blood-cerebrospinal f...

The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the interaction was studied by isothermal titration calorimetry and differential scann...