The influence of three sugar osmolytes on the refolding of guanidine hydrochloride- (GdnHCl-) denatured trehalose-6-phosphate hydrolase of Bacillus licheniformis (BlTreA) was studied by circular dichroism (CD) spectra, fluorescence emission spectra, and the recovery of enzymatic activity. These experimental results clearly indicated that sorbitol, sucrose, and trehalose at a concentration of 0....

Thermal denaturation of a-Lactalbumin in the absence and presence of various concentrations of sucrose,sorbitol, glucose and galactose as sugar osmolytes were measured by monitoring changes in the absorptions thatcarried out in a Lambd 35 UV-Vis double beam spectrophotometer at pH 6.0. These measurements gave valuesof T., (midpoint of denaturation), AH., (enthalpy change at T.), and ACp (consta...

Chemical denaturation and thermal denaturation of13-lactoglobulin A (f3 — lgA) in the absenceand presence of various concentrations sugar osmolytes and polyols were measured bymonitoring changes in the absorption coefficients at pH 2.0. It has been observed that AGD°(H20), (Gibbs free energy change in absence of denaturant at 25 °C) of f3-1gA in the presenceof 10% (w/v) Trehalose, Sucrose, Sorb...

chemical denaturation and thermal denaturation of13-lactoglobulin a (f3 — lga) in the absenceand presence of various concentrations sugar osmolytes and polyols were measured bymonitoring changes in the absorption coefficients at ph 2.0. it has been observed that agd°(h20), (gibbs free energy change in absence of denaturant at 25 °c) of f3-1ga in the presenceof 10% (w/v) trehalose, sucrose, sorb...

thermal denaturation of a-lactalbumin in the absence and presence of various concentrations of sucrose,sorbitol, glucose and galactose as sugar osmolytes were measured by monitoring changes in the absorptions thatcarried out in a lambd 35 uv-vis double beam spectrophotometer at ph 6.0. these measurements gave valuesof t., (midpoint of denaturation), ah., (enthalpy change at t.), and acp (consta...

Thermal denaturation of p-Iactoglobulin type B in the absence and pteNcno: of g arious concentrations oftrehalo5e, sucrose and sork toles sugar osmolytes and twlyols were nuyoured hy monitoring changes in theabsorption coefficients at pH 2.0. These measurements gave aliss, or I. mpdpDint of denaturation), Al-fis(enthalpy change at Ty). and ACp (consttun-pressure heal capaciit eh.iltgtii under a...

thermal denaturation of p-iactoglobulin type b in the absence and ptencno: of g arious concentrations oftrehalo5e, sucrose and sork toles sugar osmolytes and twlyols were nuyoured hy monitoring changes in theabsorption coefficients at ph 2.0. these measurements gave aliss, or i. mpdpdint of denaturation), al-fis(enthalpy change at ty). and acp (consttun-pressure heal capaciit eh.iltgtii under a...

In this report, we compared the effects on the growth of Lactobacillus plantarum of raising the medium molarity by high concentrations of KCl or NaCl and iso-osmotic concentrations of nonionic compounds. Analysis of cellular extracts for organic constituents by nuclear magnetic resonance spectroscopy showed that salt-stressed cells do not contain detectable amounts of organic osmolytes, whereas...