The C terminus of the tubulin alpha-subunit of most eukaryotic cells undergoes a cycle of tyrosination and detyrosination using two specific enzymes, a tubulin tyrosine ligase (TTL) and a tubulin carboxypeptidase. Although this enzyme cycle is conserved in evolution and exhibits rapid turnover, the meaning of this modification has remained elusive. We have isolated several NIH-3T3 derived clona...

We demonstrate an optical time-to-live (TTL) decrementing module for optical packet-switched networks. Our module acts on a standard NRZ-modulated binary TTL field within a 10 Gb/s packet and decrements it by one if the TTL is nonzero. If the TTL of the incoming packet is zero, the module signals an optical switch to drop the packet. Our technique is independent of the TTL length, does not requ...

The Transition Network is a global grassroots network that supports community-led resilience in the face of global change. This paper reports on an ethnographic study of one of its longest-running projects, Transition Town Lewes (TTL) in the United Kingdom. The aim of the study is to analyse TTL as a community. More specifically, we ask two questions: (1) what type of community is TTL? and (2) ...

This chapter presents the hybrid Temporal Trace Language (TTL) for formal specification and analysis of dynamic properties of multi-agent systems. This language supports specification of both qualitative and quantitative aspects, and subsumes languages based on differential equations and temporal logics. TTL has a high expressivity and normal forms that enable automated analysis. Software envir...

Time-to-Live data in the IP header offers two interesting characteristics: First, different IP stacks pick different start TTL values. Second, each traversed router should decrement the TTL value. The combination of both offers host and route fingerprinting options. We present the first work to investigate Internet-wide TTL behavior at carrier scale and evaluate its fit to detect anomalies, pre...

Tubulin-tyrosine ligase (TTL), the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin, has been purified from porcine brain using a purification scheme based on standard biochemical procedures. The enzyme preparation was nearly homogeneous (purity greater than 95%), was free of tubulin, and could be stored in the presence of glycerol for se...

This paper presents a way of modeling the hit rates of caches that use a time-to-live (TTL)-based consistency policy. TTL-based consistency, as exemplified by DNS and Web caches, is a policy in which a data item, once retrieved, remains valid for a period known as the “time-to-live”. Cache systems using large TTL periods are known to have high hit rates and scale well, but the effects of using ...

This paper presents a way of modeling the hit rates of caches that use a time-to-live (TTL)-based consistency policy. TTL-based consistency, as exemplified by DNS and Web caches, is a policy in which a data item, once retrieved, remains valid for a period known as the “time-to-live”. Cache systems using large TTL periods are known to have high hit rates and scale well, but the effects of using ...

BRASSINOSTEROID-INSENSITIVE 1 (BRI1) is a Leu-rich-repeat (LRR) receptor kinase that functions as a critical component of a transmembrane brassinosteroid (BR) receptor. It is believed that BRI1 becomes activated through heterodimerization with BAK1, a similar LRR receptor kinase, in response to BR signal. A yeast two-hybrid screen using the kinase domain of BRI1 identified an Arabidopsis thalia...