Aim: This study was undertaken to assess the differential expression levels of toll-like receptors (TLRs) 2, 3 and 7 in peripheral blood mononuclear cells (PBMCs) isolated from Tharparkar and Crossbred cattle belonging to different regions of India. Materials and Methods: PBMCs were isolated from blood samples of Tharparkar cattle from Indian Veterinary Research Institute (IVRI) farm (n=30); Su...

AIM The present study compared the testicular cytology and histology between crossbred (Holstein-Friesian [HF] × Tharparkar) and purebred (HF and Tharparkar) bulls to find out differences if any. MATERIALS AND METHODS Four peripubertal bulls from each breed were utilized for the study. Through percutaneous needle aspiration biopsy, Sertoli and spermatogenic cells were extracted, and morphomet...

Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism ...

Information on genetic structure and variability of livestock breeds is critical for efficient conservation plans. Here we present molecular characterization of 5 Indian grey colored cattle breeds from northern and western parts of India on the basis of 21 FAO recommended microsatellite markers. Kankrej breed has its native tract in the state of Gujarat, Tharparkar, Nagori and Mewati in Rajasth...

AIM One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na(+)/K(+)-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls cross-membrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na(+)/K(+)-ATPase, for genetic polymorphisms. MATERIALS AND METHODS A...

AIM The present investigation was undertaken to study the impact of heat stress and hypercapnia on physiological, hematological, and behavioral profile of Tharparkar and Karan Fries (KF) heifers. MATERIALS AND METHODS The animals of both the breeds of Tharparkar and KF were exposed at different temperatures and CO2 levels. Exposure conditions of 25°C, 400 ppm CO2 level, and 60% relative humid...

Abstract The Thar Desert is located at the confluence of Sindh, Gujarat, and Rajasthan. region’s folklore refers to desert as a sea through which cast warriors, merchants, adventurers traveled. Oral histories connect it with business subsistence exchanges. These tell crossing Rann Kutch (i.e., into India from Pakistan). They illustrate strong connection between Tharparkar (in Pakistani province...

AIM Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of...

The present study was conducted to quantify and compare TLR2 (toll-like receptor 2) activity in monocyte-derived macrophages of zebu (Tharparkar) and crossbred (Holstein-Friesian × Jersey × Brown Swiss × Hariana) cattle. The cells were either induced with Pam3CSK4 or kept as control. The TLR2 activity was quantified in terms of IκB-α inhibitory subunit (NFKBIA) messenger RNA (mRNA) copies...