introduction: manipulation of protein stability is important for understanding the principles that govern protein thermostability, both in basic research and industrial applications. various data mining techniques exist for prediction of thermostable proteins. furthermore, ann methods have attracted significant attention for prediction of thermostability, because they constitute an appropriate ...

background and aims: the safe, potent and effective live vaccines against measles, mumps and rubella as important childhood diseases have been available for several decades. several factors can affect the thermal stability of lyophilized vaccine. methods: in this study, the effect of residual moisture on thermal stability of 61 batches of mmr vaccine was investigated using an accelerated method...

Protein thermostability is a crucial factor for biotechnological enzyme applications. Protein engineering studies aimed at improving thermostability have successfully applied both directed evolution and rational design. However, for rational approaches, the major challenge remains the prediction of mutation sites and optimal amino acid substitutions. Recently, we showed that such mutation sites...

The need to keep vaccines cold in the face of high ambient temperatures and unreliable access to electricity is a challenge that limits vaccine coverage in low and middle-income countries (LMICs). Greater vaccine thermostability is generally touted as the obvious solution. Despite conventional wisdom, comprehensive analysis of the value proposition for increasing vaccine thermostability has bee...

we expressed and purified a recombinant p. pyralis luciferase with n-terminal his-tags. the silanized ni or cu-loaded magnetic particles were prepared and used to assemble the his-tagged p. pyralis luciferase. this enzyme immobilized on functionalized magnetic nanoparticles (mnps) via electrostatic interactions of his-tag with ni2+/cu2+ ions on the surface of mnps using simple one step method. ...

Six previously constructed and nine newly constructed Aspergillus awamori glucoamylases with multiple mutations made by combining existing single mutations were tested for their ability to produce glucose from maltodextrins. Multiple mutations have cumulative effects on glucose yield, specific activity and thermostability. No general correlation between glucose yield and thermostability was obs...

Protein thermostability information is closely linked to commercial production of many biomaterials. Recent developments have shown that amino acid composition, special sequence patterns and hydrogen bonds, disulfide bonds, salt bridges and so on are of considerable importance to thermostability. In this study, we present a system to integrate these various factors that predict protein thermost...

Recent developments in research on the stability of proteins - specifically, comparisons of the ion pairs of homologous structures - show that ion pairs potentially contribute to the thermostability of proteins. This study proposes a probabilistic Bayesian statistical method to efficiently predict the thermostability of proteins based on the properties of ion pairs. The experimental results sug...

Protein thermostability can be increased by some glycine to proline mutations in a target protein. However, not all glycine to proline mutations can improve protein thermostability, and this method is suitable only at carefully selected mutation sites that can accommodate structural stabilization. In this study, homology modeling and molecular dynamics simulations were used to select appropriat...

Protein thermostability engineering is a powerful tool to improve resistance of proteins against high temperatures and thereafter broaden their applications. For efficient protein thermostability engineering, different thermostability-classified data sources including sequences and 3D structures are needed for different protein families. However, no data source is available providing such data ...