Transmembrane transport of exogenous genes is widely investigated because high demand for gene therapy. Both carriers and cellular conditions can affect transfection efficiency. Although cell morphology has been reported to functions, the influence adhesion area spreading on remains unclear it difficult separate individual these areas during normal culture. In this study, micropatterns were pre...

Objective(s): Although viral vectors are considered efficient gene transfer agents, their board application has been limited by toxicity, immunogenicity, mutagenicity and small gene carrying capacity. Non-viral vectors are safe but they suffer from low transfection efficiency. In the present study, polyallylamine (PAA) in two molecular weights (15 and 65 kDa) was modified by alkane derivatives ...

objective(s): although viral vectors are considered efficient gene transfer agents, their board application has been limited by toxicity, immunogenicity, mutagenicity and small gene carrying capacity. non-viral vectors are safe but they suffer from low transfection efficiency. in the present study, polyallylamine (paa) in two molecular weights (15 and 65 kda) was modified by alkane derivatives ...

The development of efficient and safe carrier system to transfer DNA into cells is essential in non-viral gene therapy. The aim of the present study was to evaluate the effect of linear polyetheneimine (lPEI) (2500 Da) on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lPEI. Materials and Methods Different lipopolymers were synthesized from lPEI ...

New lipid conjugates of DNA and RNA incorporating one to four [(4-dodecylphenyl)sulfonyl]phosphoramidate or (hexadecylsulfonyl)phosphoramidate groups at internucleotidic positions near the 3? 5?-end were synthesized characterized. Low cytotoxicity their ability be taken up into cells without transfection agents demonstrated. Lipid-conjugated siRNAs targeting repulsive guidance molecules a (RGMa...

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its in CHO-K1 cells and the inhibition effect pig on PRRSV proliferation verified. The plasmid pEGFP-PoIFN-λ1 from gene fragment transfected into cells. Expression detected by fluorescence microscopy Western blotting. influence assessed. results study showed that recombinant correctly. After transfection, green f...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

the development of efficient and safe carrier system to transfer dna into cells is essential in non-viral gene therapy. the aim of the present study was to evaluate the effect of linear polyetheneimine (lpei) (2500 da) on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lpei. materials and methods different lipopolymers were synthesized from lpei an...

Total protein and DNA extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and DNA transforming activity in NIH 3T3 cell transfection assay, respectively. In comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptid...

It has been previously shown that transfection activity of cationic liposome/DNA lipoplexes delivered systemically is drastically inhibited by lipoproteins (Tandia, B. M., Vandenbranden, M., Wattiez, R., Lakhdar, Z., Ruysschaert, J. M., and Elouahabi, A. (2003) Mol Ther. 8, 264-273). In this work, we have compared the binding/uptake and transfection activities of DOTAP (N-[1-(2,3-dioleoyloxy)pr...