A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on signal amplification using horseradish peroxidase catalysed deposition of biotinylated tyramine at the sites of hybridisation. kappa and lambda light chain immunoglobulin mRNA in situ hybridisation was...

background: the excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. gum tragacanth (gt) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. despite,  its desirable prop...

The method of tyramine amplification is based on the ability of HRP to catalyze the deposition of large amounts of biotinylated tyramine in the vicinity of the antigen-antibody reaction (i.e. the cellular site which contains the peroxidase detection complex). The method is based on earlier biochemical observations on the action of peroxidase upon tyramine and other related compounds (GROSS AJ a...

two peroxidases (ec. 1.11.1.7), pod i and pod ii were purified from the roots of cultivatedraphanus sativus l. var. cicil by one step ion-exchange chromatography after fractionation by acetone. the molecular weight of these enzymes were 43000 and 41000 daltons and rz 1.2 and 2.0 for pod i and pod ii, respectively. both enzymes consisted of a single polypeptide chain on sds-page. the maximum act...

We have assigned the biosynthetic processing steps of cathepsin D to intracellular compartments which are involved in its transport to lysosomes in HepG2 cells. Cathepsin D was synthesized as a 51-kDa proenzyme. After formation of 51-55-kDa intermediates due to processing of N-linked oligosaccharides, procathepsin D was proteolytically processed to an intermediate 44-kDa and the mature 31-kDa e...

The atypical two-electron oxidation of thioanisole and its p-methyl, p-methoxy, and p-nitro analogues by horseradish peroxidase, contrary to earlier reports, stereoselectively produces the (8) sulfoxides in 6070% enantiomeric excess. Horseradish peroxidase reconstituted with 6-meso-ethylheme has little peroxidase (guaiacol oxidizing) activity, as previously reported, but exhibits increased sulf...

Horseradish peroxidase and chlorite, NaC102, are able to catalyze chlorination of monochlorodimedone to form dichlorodimedone. Catalytic amounts of horseradish peroxidase act to disproportionate chlorite forming chlorine dioxide and chloride ion. The chlorine dioxide thus formed is responsible for the chlorination of monochlorodimedone. It was previously thought (Chiang, R., Rand-Meir, T., Maki...

Since rates of tissue growth vary significantly between tissue types, and also between individuals due to differences in age, dietary intake, and lifestyle-related factors, engineering a scaffold system that is appropriate for personalized tissue engineering remains a significant challenge. In this study, a gelatin-hydroxyphenylpropionic acid/carboxylmethylcellulose-tyramine (Gtn-HPA/CMC-Tyr) p...