urease was immobilized on platinum electrode both by chemical binding and electropolymerization.the conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 m and linear dynamic range from 4.9×10-5 to 5.8×10-3 m for urea concentration when the enzyme is covalently immobilized on pt electrodes. conductometric transducers respond to the changes in ionic strength thereby le...

Urease, a nickel-containing metalloenzyme is getting remarkable attention due to diverse range of applications for mankind. Urease plays magnificent role in various field like agriculture, analytical, geological phenomena, beverage industry and an important diagnostic tool. mainly present bacteria, fungi, plants invertebrates its manifestation specific genera may open new vistas taxonomic posit...

a thermodynamic study of copper ions by jack bean urease (jbu) was carried out at two temperatures of 27 and 37?c in tris buffer (30 mm; ph=7.0) using an isothermal titration calorimetry. there is a set of twelve identical and non-interacting binding sites for copper ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 285 µm and ?15.2 kj/mol at 27?c and 3...

Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respo...

In this work, a glutathione (GSH) modified gold microelectrode was used for the covalent immobilization of urease biomolecules via the glutaraldehyde-coupling agent. The self-assembled monolayers (SAMs) onto the gold surface was investigated by using the electrochemical impedance spectroscopy measurements (EIS). Before urease grafting, a significant interaction was noticed between urea and the ...

Urease was covalently immobilized on the magnetic particles without and with polymer between urease particle. The insertion enhanced conversion from urea to bicarbonate. ureases were microparticles through covalent bonds. 150 μL of stock solution 3 μm diameter washed three times 20 mM MES rubbing at pH 6.0. results this study seem be useful in designing a reactor for recycling wastewater genera...

In this research, the interaction of hexadecyl trimahyl ammonium bromide (HTAB) with enzyme ureasehas been investigated comprehensively at different experimental conditions such as ionic strength, proteinconcentration using ion selective membrane electrode of surfactants. The obtained binding isotherms frompotentiometnc studies have been analyzed by different theories such as Wyman binding pote...

Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether RovM regulates the urease activity in Yptb was still unknown. In this study, by using qualitative and...

in this research, the interaction of hexadecyl trimahyl ammonium bromide (htab) with enzyme ureasehas been investigated comprehensively at different experimental conditions such as ionic strength, proteinconcentration using ion selective membrane electrode of surfactants. the obtained binding isotherms frompotentiometnc studies have been analyzed by different theories such as wyman binding pote...

RuII(arene) complexes have emerged as a versatile class of compounds to design metallodrugs as potential treatment for a wide range of diseases including cancer and malaria. They feature modes of action that involve classic DNA binding like platinum anticancer drugs, may covalent binding to proteins, or multimodal biological activity. Herein, we report the synthesis and urease inhibition activi...