Three simple, rapid, accurate, precise and cost-effective UV spectrophotometric methods have been developed and validated for simultaneous estimation of Escitalopram oxalate and Etizolam in tablet dosage form. The UV methods have been developed utilizing concept of standard addition utilizing 0.1 N HCl as a solvent. Method I is estimation using simultaneous equation method at 238.2 nm (λmax of ...

Spectrophotometric-dual-enzyme-simultaneous-assay (SDESA) for enzyme-linked-immunosorbent-assay (ELISA) of two components in one well is a patented platform when a special pair of labels is accessible. With microplate readers, alkaline phosphatase on 4-nitro-1-naphthylphosphate (4NNPP) served as label A; Pseudomonas aeruginosa arylsulfatase (PAAS) and acetylcholinesterase (AChE) on their substr...

We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...

PURPOSE To determine the UV absorbance of the corneal layers (epithelium, Bowman layer, stroma) in the 240- to 400-nm range. METHODS Consecutive slices (100 microm) of human cadaveric corneas were cut, and the UV absorbance of each sample was determined in a scanning spectrophotometer. In some cases the epithelium was scraped off and its absorbance measured separately. RESULTS The investiga...

Amidino-thiourea immobilized glass beads (AGB I) were prepared and used as the microcolumn packing for the flow injection (FI) on-line separation and preconcentration of Ag(I) coupled with atomic absorption spectrometry (AAS) determination. Base metal ions and anions with a concentration of 2.0 mg mL(-1) had no interference with the determination of Ag(I). The limit of detection (LOD) of Ag(I) ...

The appearance of kits for the determination of micro amounts of calcium stimulated our interest in the use of Eriochrome Blue SE for this analysis. A study of spectrophotometric curves indicates that, at pH values above 13.7, calcium will complex and cause a change of dye absorbance while magnesium does not complex with the dye. A differential spectrophotometric technic is described in this pa...

A 'two-objective, one-area' method and related equations are suggested to measure absorbance of microscopic stained objects. In such work, the measuring field invariably includes an image of the object and some clear area surrounding the image. The total intensity in the two areas is measured photometrically, using two different objectives, and substituted in the equation for absorbance. The eq...

The methanol extracts of leaves of Bauhinia acuminata (MESF) and their different fractions obtained from modified Kupchan partitioning method i.e. methanol, pet ether, carbon tetrachloride, chloroform, and aqueous soluble fractions were subjected to biological screening such as total phenolic content and antioxidant activity screening.The amount of total phenolic content differed in di...

Knowledge of the mechanism of human skin colour and measurement of melanin and blood concentration in human skin are needed in the medical and cosmetic fields. The absorbance spectrum from reflectance at the visible wavelength of human skin increases under several conditions such as a sunburn or scalding. The change of the absorbance spectrum from reflectance including the scattering effect doe...