Taking an alcohol swab of a person's forearm and analyzing it using a spectrophotometer has been shown to be a reliable method for detecting the presence of sunscreen. The aims of this study were to determine if moisturizing lotions or other non-sunscreen products influence the absorbance readings from skin swabs in a controlled setting, and to establish the cutoff point in determining the pres...

A simple, direct semi-micro method has been developed for serum iron and latent iron-binding capacity. For total serum iron, into a one cm cuvet are pipetted (a ) 1.5 ml of Na acetate buffer (pH 4.5) containing Na ascorbate and (b ) 0.3 ml of serum. The serum blank absorbance is measured at 562 nm, and 0.2 ml of ferrozine color reagent is added. After incubation (42°, 20 min), the absorbance is...

Taking an alcohol swab of a person’s forearm and analyzing it using a spectrophotometer has been shown to be a reliable method for detecting the presence of sunscreen. The aims of this study were to determine if moisturizing lotions or other non-sunscreen products influence the absorbance readings from skin swabs in a controlled setting, and to establish the cutoff point in determining the pres...

UNLABELLED Crataegus macracantha Lodd, family Rosaceae, is a very rare species in Europe, and unlike Crataegus monogyna is less investigated for pharmacologic activity. AIM To analyze the ability of the lyophilisate of extract obtained from leaves of Crataegus macracantha Lodd (single plant at the Iaşi Botanical Garden) to capture free radicals in vitro. MATERIAL AND METHODS The lyophilisat...

Three accurate, precise, sensitive and economical procedures for simultaneous estimation of Atorvastatin Calcium and Telmisartan in tablet dosage form have been developed. The methods employed were absorbance correction method (I), first order derivative spectroscopic method (II) and duel wavelength method (III). The first method employs wavelength 328 nm for direct estimation of Telmisartan wh...

Colour deconvolution is a method used in diagnostic brightfield microscopy to transform colour images of multiple stained biological samples into images representing the stain concentrations. It is applied by decomposing the absorbance values of stain mixtures into absorbance values of single stains. The method assumes a linear relation between stain concentration and absorbance, which is only ...

We have developed a simple biologically non-invasive method for determining the critical micellar concentration (CMC) of bile salts using pure naturally occurring bilirubin IX alpha monoglucuronide (BMG), an important bile pigment present in virtually all mammalian biles. This methodology employs visible absorbance spectroscopy of BMG in bile salts over a range of bile salt concentrations that ...

Standardization of a fragmented dermatophyte mycelial inoculum free of conidia for use in a broth microculture antimycotic susceptibility testing system is described. Ten clinical dermatophyte isolates were grown in submerged broth cultures at 35 degrees C. The mycelia were harvested before conidia appeared and were fragmented to 10 to 50-micron segments with a Broeck ground-glass tissue grinde...

Our current understanding of the spectral sensitivities of the mysticete whale rod-based visual pigments is based on two species, the gray whale (Eschrichtius robustus) and the humpback whale (Megaptera novaeangliae) possessing absorbance maxima determined from difference spectra to be 492 and 497 nm, respectively. These absorbance maxima values are blueshifted relative to those from typical te...

Here we report on a novel infrared sensor system for measuring the CH2-symmetric/CH2-antisymmetric stretch ratio of cell samples. Based on IR absorbance spectra of healthy and malignant breast [1], blood [2] and brain [3] cells found in literature we hypothesized the possibility of disease stage cell discrimination by only comparing a few absorbance peaks in the lipid absorbance wavelength regi...