A new method for characterizing amniotic fluid is presented. The difference in optical absorbarice between amniotic fluid diluted with distilled water or glycerol is measured. This differential approach is shown to determine the total light scattered by the surfactant-containing lamellar bodies and not to be affected by interfering chromogens. The lamellar body size, number density, and concent...

Maillard reaction (MR) was carried out in L-lysine-D-glucose (Lys-Glu) model system heated at 120°C for 0 to 10 h without pH control. Optical property (UV-Vis absorbance and fluorescence) development of MR was monitored. Antioxidant activity of maillard reaction products (MRPs) was investigated by a series of in vitro tests. The UV-Vis absorbance zero and first-order derivative spectrophotometr...

Isolation and structural elucidation of prune constituents were performed and total 10 compounds were determined by NMR and MS analyses. A novel compound was identified to be 2-(5-hydroxymethyl-2',5'-dioxo-2',3',4',5'-tetrahydro-1'H-1,3'-bipyrrole)carbaldehyde, and 7 phenolic compounds were isolated from prunes for the first time. In addition, antioxidant activity of them was evaluated on the b...

Specific absorbance (A|) is the maximum absorbance of a 1% solution over a 1-cm path length as measured by a spectrophotometer. If a reliable A| determination is available for a drug, it provides an extremely useful tool for the quantitative verification of a stock drug solution. Zaleplon was introduced into the market in 1999, and although the drug has been available for 10 years, A| has not b...

Extraction of DNA is very important nowadays in bio-molecular researches. Extracted DNA should be purified and the quality of DNA should also be very high. The objective of the study was to develop a simple efficient method to isolate DNA from the rice varieties in an open laboratory environment, and to eliminate the usage of expensive chemicals and tools. The DNA extraction methods developed b...

Bilirubin, the yellow substance usually responsible for neonatal jaundice, is currently monitored invasively or by observing/measuring skin colour. This paper investigates the feasibility of monitoring serum bilirubin concentration using light absorbance in a similar fashion to pulse oximetry. The light absorbance of bilirubin is shown to be sufficiently different to haemoglobin to in theory al...

The methods of (14)C-2-deoxyglucose ((14)C-2DG) metabolic mapping and optical intrinsic signal (OIS) imaging were used to evaluate the response evoked in the contralateral primary somatosensory receiving areas (SI and SII) of anesthetized cats by either 25 Hz ("flutter") or 200 Hz ("vibration") sinusoidal vertical skin displacement stimulation of the central pad on the distal forepaw. Unilatera...