In our previous study, we compared the two α-amylase enzymes from Bacillus sp.KR8104, BKA∆(N44) and BKA∆(N44C193) which is the secreted form of it. The results indicated that the presence of 193 amino acids propeptide in the C-terminal of BKA∆(N44) changed its enzymatic parameters like an uncompetitive inhibitor in comparison to BKA∆(N44C193). In the present study, we cloned the DNA sequence of...

We have investigated the molecular basis of hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency in a patient who presented with the Lesch-Nyhan syndrome. A catalytically incompetent form of HPRT has been isolated from this patient's erythrocytes and lymphoblasts. This enzyme variant, which we have termed HPRTKinston, is indistinguishable from the normal enzyme in terms of its intra...

The nanocomposites of poly (Acryl amide-co-Acrylic acid) with different contents of NaAzeolite were prepared using ammonium persulfate as an initiator and N, N′-methylenebisacrylamide as the crosslinker. The morphology was characterized by SEM and the structure ofnanocomposite materials was studied with XRD and FT-IR that showed the interaction betweenporous materials and poly (AAm-co-Aac). The...

In the present study, activated bleaching earth was used as clay adsorbent for an investigation of the adsorbability and adsorption kinetics of acid dyes (i.e., acid orange 51, acid blue 9, and acid orange 10) with three different molecular sizes from aqueous solution at 25 degrees C in a batch adsorber. The rate of adsorption has been investigated under the most important process parameters (i...

Penicillic acid, a secondary metabolite first isolated from Penicillium puberuluml, has since been shown to be produced by a variety of fungi. The compound possesses antimicrobial activity2,3, is toxic to mammals4 , and has proved carcinogenic to rats5• KURTZMAN AND CIEGLER6 found that in blue-eye-diseased com caused by Penicillium martensii, large quantities of penicillic acid are produced whe...

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...

The strength of the low-barrier hydrogen bond in hydrogen maleate in the gas phase was investigated by low-temperature photoelectron spectroscopy and ab initio calculations. Photoelectron spectra of maleic and fumaric acid monoanions (cis-/trans-HO(2)CCH=CHCO(2)(-)) were obtained at low temperatures and at 193 nm photon energy. Vibrational structure was observed for trans-HO(2)CCH=CHCO(2)(-) du...

The efficiency and feasibility of organic acids, especially formic and benzoic acids in the feeding of fur animals was investigated in five experiments, where the main emphasis was focused on the metabolism and rate of elimination of these preservatives, in order to assess practical dietary recommendations. Experiments were carried out with mink (Mustela vison), blue foxes (Alopex lagopus) and ...