CHIRALPAK QN-AX and QD-AX are anion exchanger chiral stationary phases providing specifi c enantioselectivity for acidic compounds. In this Application Note, we demonstrate that the application of these columns can be extended to supercritical fl uid chromatography (SFC) for enantiomer separation of acidic compounds. Using the advantages of the advanced technology of the Agilent 1260 Infi nity ...

Transcriptional regulatory mechanisms are fundamentally similar in eukaryotic organisms [1,2]. Components of the RNA polymerase II (Pol II) machinery are highly conserved and, in some cases, functionally interchangeable. Transcriptional activators with similar DNA-binding specificities are present from yeast to human, and acidic activation domains stimulate transcription across a wide range of ...

ARTICLE HISTORY Received 8 May 2017 Accepted 15 May 2017 ABSTRACT Much contemporary evidence underscores the pathophysiological importance of Ca2C handling by acidic organelles such as lysosomes. Whereas our knowledge of how Ca2C is released from these acidic Ca2C stores (the ‘outs’) is advancing, we know relatively little about how Ca2C uptake is effected (the ‘ins’). Here I highlight new work...

The relative contributions of external and intracrystalline acidic sites of small pore H-RHO zeolite for the selective synthesis of methylamines from methanol and ammonia have been studied. Nonselective surface reactions which produce predominantly trimethylamine can be eliminated by "capping" the external acidic sites with trimethylphosphite (TMP) and other reagents, thus improving the selecti...

Glutathione S-transferases are a group of drug metabolising and detoxification enzymes. We have studied the distribution of four isoenzymes, acidic, basic, neutral, and microsomal GST in human liver, gall bladder, and small and large intestinal epithelium by immunohistochemistry. Antibodies were raised in rabbits to purified GST subunits and several formalin fixed paraffin sections of each huma...

Acidic gel electrophoresis of ricin subunits Abstract Ricin is a potent toxic castor bean glycoprotein comprising of two subunits, A chain and B chain. We studied ricin subunits in an acidic electrophoresis system followed by electro blotting. To the best of our knowledge, there is no study reported for characterization of ricin subunits using acidic native PAGE. Sample preparation steps for ac...

Capsaicin evokes a membrane current in trigeminal ganglion neurons that is increased substantially in a moderately acidic extracellular environment. Using excised outside-out membrane patches, we studied the mechanism by which protons enhance the sustained response to capsaicin. In the absence of capsaicin, extracellular exposure to a moderately acidic physiological solution (pH 6.6) did not re...

A simple and precise micro-method for measurement of daily fecal excretion of neutral and acidic sterols has been developed which utilizes sitostanol (24-ethyl-5 alpha-cholestane-3 beta-ol) as fecal flow and recovery marker. Extractions of sterols were performed from 50 microliters of fecal homogenate (feces-water 1:1), and analyses of neutral and acidic sterols were carried out by gas-liquid c...

Highly purified acidic (pI 4.9) and basic (pI 8.7) phospholipases A2 from snake (Agkistrodon halys blomhoffii) venom hydrolyzed approximately 20% and 60%, respectively, of the phosphatidylcholine (PC) of intact human erythrocytes prior to hemolysis. Sequential use of the acidic enzyme followed by the basic phospholipase A2 or vice versa manifested a characteristic PC hydrolysis pattern. For exa...