The feature of intact human erythrocytes and erythrocyte white ghosts is a unique sialidase activity with acidic optimal pH (acidic sialidase). The treatment of white ghosts with mildly alkaline isotonic solutions at 37 degrees C, like that used to produce resealed ghosts, is accompanied by the expression, together with the acidic sialidase, of a novel sialidase with a pH optimum of 7.2 (neutra...

As two important physico-chemical parameters, the acidic and alkaline residues of protein are of evident significance for the evaluation of protein properties and the design of relevant separation and analysis. However, there is still no electrophoretic method used for the direct detection of free acidic and alkaline residues of protein. Herein, we developed the concepts of moving reaction boun...

The effects of an acidic condition (pH 6.5) on WAF1 gene expression and p53 accumulation was investigated in human glioblastoma cells with different p53 statuses. WAF1 and p53 accumulation after treatment in acidic conditions was observed in A-172 cells carrying the wild-type p53 gene but not in T98G cells carrying the mutant p53 gene. Northern blot analysis showed that WAF1 gene activation by ...

The gas phase acidities of a series of uracil derivatives (1-methyluracil, 3-methyluracil, 6-methyluracil, 5,6-dimethyluracil, and 1,3-dimethyluracil) have been bracketed to provide an understanding of the intrinsic reactivity of uracil. The experiments indicate that in the gas phase, uracil has four sites more acidic than water. Among the uracil analogs, the N1-H sites have deltaH(acid) values...

Acidic deposition is comprised of sulfuric and nitric acids and ammonium derived from atmospheric emissions of sulfur dioxide, nitrogen oxides, and ammonia, respectively. Acidic deposition has altered soil through depletion of labile pools of nutrient cations (i.e. calcium, magnesium), accumulation of sulfur and nitrogen, and the mobilization of elevated concentrations of inorganic monomeric al...

This study aimed to investigate the effects of mixed biochar on the nitrification rate in acidic soils. A 15N tracer experiment with (15NH4)2SO4 was conducted to determine the nitrification rates of four acidic agricultural soils with pH 4.03–6.02 in Yunnan Province, Southern China. The accumulation of 15N-NO3− and nitrification rates decreased with the addition of biochar at the end of incubat...