A concise method for the synthesis of heterocycle-fused naphthoquinones such as naphtho[2,3-b]-furan-4,9-dione, 1H-benz[f]indole-4,9-dione, and naphtho[2,3-b]thiophene-4,9-dione was developed. This method employed Sonogashira coupling and tandem addition-elimination/intramolecular cyclization, and it enabled the preparation of versatile heterocycle-fused naphthoquinones from one substrate.

Due to purely steric effects of acridine units, acenaphthylene-1,2-diyldi(9-acridine) has a long C=C bond [1.3789(19) Å] while maintaining its sp(2) hybridized nature and bond order.

From reactions between glycolide or lactide (4 equiv.) with 4-dimethylaminopyridine, DMAP (1 equiv.) and NaBPh(4) (1 equiv.) in benzene at 70 degrees C the cyclic ester adducts (CH(2)C(O)O)(6)NaBPh(4) and (CHMeC(O)O)(6)NaBPh(4) are formed respectively. The structures of the salts Na[(S,R,S,R,S,R)-(CH(3)CHC(O)O)(6)](2)BPh(4).CH(3)CN and (CH(2)C(O)O)(6)NaBPh(4).(CH(3)CN)(2) are reported. The cycl...

Phytochemical profiling was undertaken on the crude extracts of Drosera magna to determine nature chemical constituents present. In total, three new flavonol diglycosides (1–3), one flavan-3-ol glycoside (4), and 12 previously reported compounds (5, 9), (15), flavanone (8), 1,4-napthoquinone (6, 7, 13, 14), 2,3-dehydroxynapthalene-1,4-dione (10–12), phenolic acid (16) structure classes were iso...

Synthesis and characterization of a new macrocyclic compound, composed by a triethylentetraamine chain linking the 4 and 5 positions of an acridine moiety, are reported. The molecule, devised as a fluorescent chemosensor for anions, has revealed an intriguing pH-dependent spectroscopic behavior, whose features are the specific object of this article. Ligand protonation in aqueous solution has b...

Fluorescent DeltapH and DeltaPsi indicators have been screened for the non-invasive monitoring of bioenergetic processes in whole cells of the cyanobacterium Synechocystis sp. PCC 6803. Acridine yellow and Acridine orange proved to be the best DeltapH indicators for the investigation of thylakoid and cytoplasmic membrane energization: While Acridine yellow indicated only cytosolic energization,...

Specimens of cells derived from tumors of the human female genital tract plus normal cells as standards have been divided into aliquots and stained according to acridine orange or pararosanilin:Feulgen procedures. Acridine orange-stained cells were slit-scanned for 535 nm nuclear fluorescence; Feulgen-stained cells were comb-scanned for 580 nm nuclear absorbance. For each specimen examined, the...

INTRODUCTION: Micronuclei (MN) are microscopically visible round or oval cytoplasmic chromatin masses in the extra nuclear vicinity, originating from aberrant mitosis, chromatic fragments or whole chromosomes. The local factors like smoking exerts an influence on oral mucosa cells which are reflected as cellular changes resulting in the formation of MN. AIM: To evaluate and compare the MN index...

The gene encoding the putative reductase component (KshB) of 3-ketosteroid 9α-hydroxylase was cloned from Rhodococcus equi USA-18, a cholesterol oxidase-producing strain formerly named Arthrobacter simplex USA-18, by PCR according to consensus amino acid motifs of several bacterial KshB subunits. Deletion of the gene in R. equi USA-18 by a PCR-targeted gene disruption method resulted in a mutan...

Nucleic acids can undergo dynamic conformational changes associated with the regulation of biological processes. A molecule presenting larger affinities for alternative structures relative to a duplex is expected to modify such conformational equilibria. We have previously reported that macrocyclic bis-acridine binds preferentially to single-stranded regions, especially DNA hairpins, due to ste...