The insulin-regulated glucose transporter GLUT4 is a key modulator of whole body glucose homeostasis, and its selective loss in adipose tissue or skeletal muscle causes insulin resistance and diabetes. Here we report an RNA interference-based screen of protein kinases expressed in adipocytes and identify four negative regulators of insulin-responsive glucose transport: the protein kinases PCTAI...

The present study was carried out to determine the fatty acid profile of rainbow trout (Oncorynchus mykiss) from three Slovenian fish farms; Zalog, Želimlje and Povodje. Fatty acids composition determined on a gas chromatograph with flame ionization detector (GC-FID). results showed that farming conditions have significant influence trout. predominant saturated (SFA) palmitic acid, oleic main m...

OBJECTIVES Arachidonic acid is a second messenger which activates protein kinase C (PKC) and is released from the heart during ischaemic preconditioning. The purpose of this study was to examine the effect of arachidonic acid on activation of PKC in cardiac myocytes and the cellular consequences. METHODS Neonatal rat cardiac myocytes were isolated and maintained in culture. Arachidonic acid-i...

Low birth weight (LBW) babies are associated with neonatal morbidity and mortality at increased risk for noncommunicable diseases (NCDs) in later life. However, the molecular determinants of LBW not well understood. Placental insufficiency/dysfunction is most frequent etiology fetal growth restriction resulting placental epigenetic processes suggested to be important regulators pregnancy outcom...

The fatty acid contents of wild and cultured Australian adult blacklip abalone, Haliotis rubra, were analysed by gas liquid chromatography. Wild abalone contained significantly higher levels of total n-3 polyunsaturated fatty acids (PUFA), eicosapentaenoic acid (20:5n-3), docosapentaenoic acid (22:5n-3) and alpha-linolenic acid (18:3n-3) than cultured abalone (P<0.05). The predominant n-3 PUFA ...

In the present study, the cytotoxicity of palmitic, stearic, oleic, linoleic, arachidonic, docosahexaenoic and eicosapentaenoic acids on a macrophage cell line (J774) was investigated. The induction of toxicity was investigated by changes in cell size, granularity, membrane integrity, DNA fragmentation and phosphatidylserine externalization by using flow cytometry. Fluorescence microscopy was u...

Human genome-wide association studies have revealed a large number of genomic loci linked to cardiometabolic disease traits. However, for many of the gene variants identified, it remains unknown how they functionally influence the disease phenotype or whether these genes represent new therapeutic targets. Single-nucleotide polymorphisms in FADS1, which encodes Δ-5 fatty acid desaturase, have be...

WISP2 is a novel adipokine, most highly expressed in the adipose tissue and primarily in undifferentiated mesenchymal cells. As a secreted protein, it is an autocrine/paracrine activator of canonical WNT signaling and, as an intracellular protein, it helps to maintain precursor cells undifferentiated. To examine effects of increased WISP2 in vivo, we generated an aP2-WISP2 transgenic (Tg) mouse...