the present study was carried out to evaluate the effects of dietary nano-structured zeolite (nz) levels on reduction of aflatoxin b1 (afb1) in the diet of rainbow trout (oncorhynchus mykiss) for 56 days. for this purpose, 270 fish (23 ± 3.7 g) were randomly distributed into 6 groups including c (control), nz0.5 (basic ration + 0.5 % nano-structure zeolite), nz1 (basic ration + 1 % nano-structu...

Five experimental groups were used to evaluate the efficiency of sodium bentonite (BNT) and ascorbic acid (AA) in reducing the toxicity of aflatoxin B1 in ElSalam chicks (Egyptian local chicks) diets. A total number of 450 unsexed one-day old chicks were wing banded, weighted and randomly assigned to 5 groups of five replicates (18 chicks/replicate). The experimental groups were as follow: 1) b...

We have examined the time course and patterns of covalent aflatoxin B1:DNA adducts produced within the ribosomal RNA gene sequences isolated from the liver nuclear DNA of aflatoxin B1-treated animals. Liver nuclear DNA was initially enriched in ribosomal DNA by cesium salt density centrifugation, and incubated under alkaline conditions to stabilize bound aflatoxin B1-DNA moieties. Alkali-treate...

Five monoclonal antibodies were obtained after fusion of mouse P3 x 63 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with aflatoxin B1-adducted DNA complexes with methylated bovine serum albumin. Selected hybridomas were found to produce monoclonal antibodies specific for modified DNA containing both the 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 and the...

Clinical isolates of fifty strains of A. fumigatus and 30 strains of A. flavus from immmunocompromised patients from the hematological unit were analyzed for mycotoxin production and compared with the same number of environmental isolates (from soil, compost, and air). Only 9 (18%) strains of A. fumigatus produced gliotoxin in a mean concentration 2.22 mg mL-1 (range 0.5-5 mg mL-1). Aflatoxin B...

1 A series of experiments were conducted to investigate the effects of dietary retinol status on chickens ingesting aflatoxin B1. The effects of dietary supplementation with biotin and alpha-tocopherol were also examined. 2. Aflatoxin B1 levels greater than 1 mg/kg diet had a detrimental effect on 'liveability', body-weight gain, food intake and food conversion efficiency. When fed for more tha...

Introduction: Contamination of foodstuffs with fungi and the toxins produced by them is a very important problem in human and animal feeding. In most villages around Shiraz, in small-scale dairy farms, dry leftover bread which is mostly contaminated with fungi is used as a part of food ration for animals. The thin-layer chromatographic method which was used for determination of aflatoxin. Inges...

AFLATOXIN B1, a carcinogenic metabolite of the fungus Aspergillus flavus, is acutely toxic to a number of species, including rats. Male rats (LD50 7-2 mg./kg.) are more susceptible to the acute oral effects of aflatoxin than are females (LD50 17.9 mg./kg.) (Butler, 1964). Aflatoxin B1 is metabolised to aflatoxin M1 which is present in the blood and urine of dosed rats (Butler and Clifford, 1965...

Monoclonal antibodies specific for aflatoxin B1, aflatoxin B2, aflatoxin M1, and the major aflatoxin-DNA adducts were obtained following fusion of mouse SP-2 myeloma cells with spleen cells of mice immunized with aflatoxin B1 covalently bound to bovine gamma globulin. The aflatoxin-modified protein used to immunize mice was produced chemically by activating aflatoxin B1 to a 2,3-epoxide derivat...

introduction: contamination of foodstuffs with fungi and the toxins produced by them is a very important problem in human and animal feeding. in most villages around shiraz, in small-scale dairy farms, dry leftover bread which is mostly contaminated with fungi is used as a part of food ration for animals. the thin-layer chromatographic method which was used for determination of aflatoxin. inges...