A microarray analysis was performed to study the effect of varying combinations of water activity and temperature on the activation of aflatoxin biosynthesis genes in Aspergillusflavus grown on YES medium. Generally A. flavus showed expression of the aflatoxin biosynthetic genes at all parameter combinations tested. Certain combinations of a(w) and temperature, especially combinations which imp...

The role of microbial secondary metabolites in the ecology of the organisms that produce them remains poorly understood. Variation in aflatoxin production by Aspergillus flavus is maintained by balancing selection, but the ecological function and impact on fungal fitness of this compound are unknown. We hypothesize that balancing selection for aflatoxin production in A. flavus is driven by inte...

It has been reported that hypophysectomy protects against the hepatocarcinogenic action of aflatoxin B in rats. A study has been made of the effects of aflatoxin B, and hypophysectomy on the livers of rats of different ages. Four groups of animals have been used, weanlings, small and large hypophysectomized rats, and adult intact rats. Ingestion of 4 ppm aflatoxin B, for 4 to 5 weeks resulted i...

Using semisolid methylcellulose HAT medium selection and non-competitive and competitive indirect enzyme-linked immunosorbent assay, three hybridomas that secreted antibodies for aflatoxin G(1) were selected after fusion of mouse SP2/0 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with the conjugate of aflatoxin G(1) (AFG(1)) and bovine serum albumin. The afl...

Kinetic pulse-labeling of aflatoxin pathway compounds was carried out in Aspergillus parasiticus, beginning with radioactive acetate. Norsolorinic acid, averufin, versicolorin A, and sterigmatocystin (all known as compounds which can be incorporated into the aflatoxin molecule) were radiotraced to follow their order of appearance. Aflatoxin species B1, B2, G1, and G2 were included. Norsolorinic...

This paper reports the evidence for the existence of multiple sites of action of aflatoxin B, in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B, injection (0.3 mg/lOO g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

Nowadays, the separation of pistachios which seem to have aflatoxin is done according to their organoleptic physical qualities like color and etc. As these methods are not  fully practical, and there is always a possibility of error and also the conditions like the wrong time harvest, the transportation of picked pistachios, the delay on the processing cause some contaminations in pistachios wh...

Background and Purpose :Aflatoxin M1, in milk and dairy products, is classified by the International Agency for Research on Cancer as human carcinogens (class 2B). The aim of this study was to evaluate aflatoxin M1 contamination level in dairy products from Iranshahr city, Iran, using ELISA technique. Materials and Methods: In our study, 87 samples of milk, white cheese, yoghurt, and butter...

Aflatoxins are highly toxic compounds that are produced as secondary metabolites by some Aspergillus and Emericella species. Despite huge progress on aflatoxin research during the past 50 years, we have witnessed many cases of food contamination with aflatoxins all over the world. Aflatoxins have mutagenic and carcinogenic effects and they are capable of inducing hepatocellular carcinoma in hum...