Both atomic force microscopy (AFM) experiments and molecular dynamics (MD) simulation are carried out to investigate atomic stick-slip friction by sliding a Pt tip on an Au substrate. Efforts are taken to match the conditions for AFM experiment and MD simulation as closely as possible. The results show that AFM and MD provide consistent energetic parameters, which suggests that MD simulations c...

Recent development of atomic force microscopy (AFM) applications in imaging living cells is reviewed, focusing on technical progress and application advancements made in the following major areas: (i) high-resolution imaging of cellular structures, (ii) real-time monitoring of cellular dynamic processes, and (iii) detecting micromechanical properties of the cell. Technical and experimental diff...

A method is presented to indirectly measure and shorten carbon nantoubes grown from the tips of atomic force microscope (AFM) probes. The measurement component exploits the nanotubes ability to elastically buckle and requires only those signals and actuators available on a standard AFM. The shortening operation is facilitated by electric arcing using a conducting niobium substrate. The shorteni...

We used an atomic force microscope (AFM) to image samples immersed in a fluid in order to study the dynamic behavior of the membranes of living cells. AFM images of cultured cells immersed in a buffer were obtained without any preliminary preparation. We observed surface changes and displacements which suggest that the cells were still alive during the measurements. Some membrane details imaged...

Quantifying the tip-sample interaction forces in amplitude-modulated atomic force microscopy (AM-AFM) has been an elusive yet important goal in nanoscale imaging, manipulation and spectroscopy using the AFM. In this paper we present a general theory for the reconstruction of tip-sample interaction forces using integral equations for AM-AFM and Chebyshev polynomial expansions. This allows us to ...

Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three-dimensional images of biological structures, including biomolecules, lipid films, 2D protein crystals and cells, under physiological ...

AFM allows the visualization of biological samples under physiological solution conditions, at high spatial resolution. However, captured images are limited to snapshots because it takes at least 30 seconds to capture an image, whereas biological phenomena are highly dynamic. To overcome this limitation, my group has developed high-speed AFM. The dynamic processes and structural dynamics of pro...

sors capable of resolving forces up to 490pN with a resolution of O.OlpN in z, and up to 900pN with a resolution of 0.24~N in I, in the oassive mode, Electrostatic microactuators are microsc~py (AFM) techniques, mere are four limitations restricting AFM use in cellular force measurement during .~~ inkgrated to the force t, operate in manipulation. First, different types of end-effectors such as...