This paper presents our work in developing an autonomous micromanipulation system. The originality of our system is that it takes advantage of adhesion forces to grip micro-objects using an AFM (Atomic Force Microscopy) probe. A theoretical analysis of rolling conditions is carried out in order to achieve precise release of an object picked-up by adhesion. Vision control, based on the specifici...

PURPOSE To demonstrate the feasibility of measuring the elasticity of intact crystalline lenses using atomic force microscopy (AFM). METHODS AFM elasticity measurements were performed on intact lenses from 18 fresh cynomolgus monkey cadaver eyes (4-10 years old, <1 day postmortem) that had been left attached to their zonule-ciliary body-sclera framework. The eyes were prepared by bonding a pl...

The work investigates the adhesive/cohesive molecular and physical interactions together with nanoscopic features of commonly used orally disintegrating tablet (ODT) excipients microcrystalline cellulose (MCC) and D-mannitol. This helps to elucidate the underlying physico-chemical and mechanical mechanisms responsible for powder densification and optimum product functionality. Atomic force micr...

How cell envelope constituents are spatially organised and how they interact with the environment are key questions in microbiology. Unlike other bioimaging tools, atomic force microscopy (AFM) provides information about the nanoscale surface architecture of living cells and about the localization and interactions of their individual constituents. These past years have witnessed remarkable adva...

In this study, we present a new technique called correlative atomic force and transmission electron microscopy (correlative AFM/TEM) in which a targeted region of a sample can be observed under AFM and TEM. The ultimate goal of developing this new technique is to provide a technical platform to expand the fields of AFM application to complex biological systems such as cell extracts. Recent adva...

Cationic AntiMicrobial Peptides (CAMPs) are specific antibiotic agents with strong bactericidal properties and are considered as a one of the alternative ways to treat the resistant forms of bacteria (Rautenbach & Hastings, 1999; Hancock & Patrzykat, 2002; Alves et al., 2010). CAMPs have been isolated from single-cell microorganisms, plants, amphibians, birds, fish, and mammals (Hancock & Diamo...

Charge carrier transport through the probe-sample junction can have substantial consequences for outcomes of electrical and electromechanical atomic-force-microscopy (AFM) measurements. For understanding physical processes under probe, we carried out conductive-AFM (C-AFM) measurements local current-voltage (I-V) curves as well their derivatives on samples a mixed ionic-electronic conductor Li1...