BACKGROUND The purpose of this study was to investigate the genesis of the Austin Flint murmur using Doppler and echocardiographic imaging. METHODS AND RESULTS A total of 51 patients having significant aortic insufficiency and an anatomically normal mitral valve were evaluated. They were divided into two groups; 30 patients had an audible Austin Flint murmur (AFM+) and 21 did not (AFM-). All ...

in this paper, surface roughness of ht29 cancerous is determined using topography images obtained by atimic force microscopy (afm). the mean radiuses of cell and substrate surface asperities are determined, based on results of conducted roughness analysis and rabinovich roughness theory. these values have been applied in the equations of rough particle nanomanipulation dynamic model and the amo...

The scaling-index method (SIM) is a novel tool for image processing in scanning-probe microscopy. Originating from the theory of complex systems, the SIM can be used in order to extract structural information from arbitrary data sets. This method can readily be applied to the analysis of digital atomic-force microscopy (AFM) images. Especially for biomedical diagnostics, where genetic material ...

The analysis of nanomechanical properties is becoming an increasingly useful tool in a large variety of fields, ranging from biology to polymer science. The Atomic Force Microscope, AFM, can bridge the information about morphology, obtained with outstanding resolution, to local mechanical properties. When performing an AFM nanoindentation, the rough force curve, i.e. the plot of voltage output ...

We report here the highly sensitive detection of protein in solution at concentrations from 10-15 to 10-18 m using the combination of atomic force microscopy (AFM) and mass spectrometry. Biospecific detection of biotinylated bovine serum albumin was carried out by fishing out the protein onto the surface of AFM chips with immobilized avidin, which determined the specificity of the analysis. Ele...

Considered as topographic reliefs, grayscale images can be decomposed into a number of peaks that can be stored in the data structure of a tree. This decomposition is called the peak analysis of grayscale images in this paper. Its mathematical definition and its fast algorithm based on the watershed transform are both presented. Due to its intuitive definition, there is a wide range of applicat...

Recently, the isolation and biochemical analysis of DNA at the single-molecule level has been recognized as very important for genetic research and clinical analysis. A unique technique for the positioning, dissection, and isolation of single DNA molecules using atomic force microscopy (AFM) has been demonstrated. Full-length genome DNA molecules were first deposited and stretched by a modified...

Coated carbide inserts are considered vital components in machining processes and advanced functional surface integrity of inserts and their coating are decisive factors for tool life. Atomic Force Microscopy (AFM) implementation has gained acceptance over a wide spectrum of research and science applications. When used in a proper systematic manner, the AFM features can be a valuable tool for a...