The incidence of contamination of aflatoxin M1 (AFM1) in milk samples collected from the Jordanian market was investigated by using the competitive enzyme linked immunosorbent assay (ELISA) technique. A total of 175 samples were collected during 2014-2015. All tested samples were contaminated with various levels of AFM1 ranging from 9.71 to 288.68 ng/kg. The concentration of AFM1 in 66% of fres...

Aflatoxin M1 (AFM1) residues in milk are regulated in many parts of the world and can cost dairy farmers significantly due to lost milk sales. Additionally, due to the carcinogenicity of this compound contaminated milk can be a major public health concern. Thirty-four lactating dairy cows were utilised to investigate the relationship between somatic cell counts (SCC), milk yield and conversion ...

Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and ...

Aflatoxins are natural toxic compounds produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Due to higher temperature and humidity, fungi are continuously grown and contaminated the cereals and cereal by-products with aflatoxin B1 (AFB1). Dairy cows fed AFB1 contaminated feeds can produce milk contaminated with aflatoxin M1 (AFM1). Therefore, the aim of this study was to in...

In the present work the results of a survey conducted in Sardinia Region on Aflatoxin M1 (AFM1) contamination in milk of small ruminants from 2005 to 2013 are reported. A total of 517 sheep and 88 goat milk samples from bulk tank, tank trucks and silo tank milk were collected. Analyses were performed by the Regional Farmers Association laboratory using high-performance liquid chromatography fol...

OBJECTIVES Aflatoxin M1 (AFM1)-contaminated dairy products pose serious human health risks, causing liver and renal failure if consumed. They are also related to decreased milk and egg production in infected animals. This study investigated the AFM1 contamination levels in cheeses sold in Isfahan province, Iran, by enzyme-linked immunosorbent assay (ELISA). METHODS A total of 100 white cheese...

This study was conducted to determine the AFM1 levels in Egyptian raw milk, soft cheese and AFB1 in table eggs marketed in Kafrelsheikh city markets. Aflatoxin content was measured using microtiter-plate enzyme-linked immunosorbent assay (ELISA). The Test Kits, RIDASCREEN ELISA of Biopharm AG that used is based on competitive enzyme immunoassay for the quantitative analysis of Aflatoxin AFM1 an...

Aflatoxin M1 (AFM1) is an important mycotoxin frequently found in milk and in dairy products. It is a minor metabolic product of Aspergillus flavus and A parasiticus. However, it occurs in dairy products as a metabolite formed in cows from aflatoxin B1 contained in animal feeds. In cheese production, AFM1 distributes between curd and whey, being present in products derived from cheese whey proc...

The objective of this study was to evaluate the occurrence of aflatoxin M1 (AFM1) in raw milk samples from 18 dairy farms in the Yangtze River Delta region during four different seasons. A total of 72 tank milk samples was collected with 18 samples for each season. Milk AFM1 was detected using LC-MS/MS. The AFM1 was detected in 43 milk samples (59.7%) ranging in concentration from 10 to 420 ng/...

An ultra-sensitive sandwich ELISA was developed for detection of AFM1 in milk. The assay involved the immobilization of rat monoclonal antibody of AFM1 in 384 microtiter plate to capture AFM1 antigen. This was detected by tracer secondary rabbit poly-clonal antibody labelled with horseradish peroxidase upon addition of a luminol-based substrate. Milk samples with different fat percentage were a...