A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for detecting aflatoxin M1 (AFM1) was developed. To improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used to enhance peroxidase-induced CL. The concentrations of the coating anti-AFM1 antibody and the conjugate of AFB1 with h...

The potent hepatotoxin and carcinogen aflatoxin B1 (AFB1) is a common mycotoxin contaminant of grains used in animal feeds. Aflatoxin M1 (AFM1) is the major metabolite of AFB1 in mammals, being partially excreted into milk, and is a possible human carcinogen. The maximum permitted concentration of AFM1 in cows' milk is 0.05 μg/kg in Israel and the European Union. Since milk yield and the carry-...

As part of the certification campaign of three candidate reference materials for the determination of aflatoxin M1 (AfM1) in whole milk powders, homogeneity, short- and long-term stability tests of naturally contaminated milk powders have been performed. The homogeneity of two AfM1-contaminated milk powders was studied by taking samples at regular intervals of the filling sequences and analysin...

One Health involves the multifaceted environment-animal-human web: nevertheless, the role of toxicological issues has yet to be fully explored in this context. Aflatoxin B1 (AFB1) contamination of feeds is a risk for the health of several farm animals, including fishes; milk is the only food of animal origin where a significant feed-food carry over may occur. The main AFB1-related compound pres...

BACKGROUND Clinoptilolite is a natural zeolite with high adsorption capacity for polar mycotoxins such as aflatoxins. The efficacy of clinoptilolite in ameliorating the toxic effects of aflatoxicosis has been proven in monogastric animals, but there is no such evidence for ruminants. The aim of this study was to evaluate, under field conditions, whether the dietary administration of clinoptilol...

Aflatoxins are a group of mycotoxins produced by certain species of Aspergillus. These molds grow on a variety of food and feed commodities and produce aflatoxins under appropriate temperature and humidity (Jay et al., 2005). Aflatoxin B1 (AFB1) is the most potent hepatocarcinogen of this group of mycotoxins. Aflatoxin M1 (AFM1) is a hydroxylated metabolite of AFB1 and is secreted in the milk o...

conclusions the prepared antibody can be used for the development of an elisa kit to assay afm1 in milk and other biological fluids. background aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the aspergillus group, which are highly toxic to animals and humans. objectives since immunoassay is a simple and rapid method for the analysis of many toxic s...

During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 μg/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chroma...

The objective of this experiment was to monitor plasma levels of aflatoxin B1 (AFB1) aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and aflatoxin M1 (AFM1) in lactating dairy cows fed afltaoxin contaminated corn. Seven lactating Holstein cows were given a bolus of a naturally contaminated corn meal assuring an intake of 4.9mg AFB1, 1.01mg AFB2, 10.63mg AFG1 and 0.89mg AFG2. Vitam...

Background: In Nigeria, there is paucity of information on exposure to aflatoxin by lactating women and children. Aflatoxin (AFs) are potent carcinogens that are produced as secondary metabolites of strains of Aspergillus parasiticus and Aspergillus flavus that grow on important food crops such as groundnuts, maize and other oilseeds .Present study reports on the aflatoxin exposure of lactating...