background: lectins the multivalent carbohydrate binding proteins are also employed to ascertain differentiation and characterization of the specific cell surface carbohydrate ligands/ receptors of different cell types including spermatozoa and posses novel biomedical importance. objective: sf50 the sialic acid specific lectin was employed on goat cauda epididymal spermatozoa to investigate its...

We have examined the carbohydrate specificity of bindin, a sperm protein responsible for the adhesion of sea urchin sperm to eggs, by investigating the interaction of a number of polysaccharides and glycoconjugates with isolated bindin. Several of these polysaccharides inhibit the agglutination of eggs by bindin particles. An egg surface polysaccharide was found to be the most potent inhibitor ...

MODIFICATIONS IN RABBIT SPERM PLASMA MEMBRANES DURING EPIDIDYMAL PASSAGE AND AFTER EJACULATION WERE INVESTIGATED BY USED OF THREE LECTINS: concanavalin A (Con A); Ricinus communis I (RCA(I)); and wheat germ agglutinin (WGA). During sperm passage from caput to cauda epididymis, agglutination by WGA drastically decreased, and agglutination by RCA(I) slightly decreased, although agglutination by C...

We have examined the carbohydrate specificity of bindin, a sperm protein responsible for the adhesion of sea urchin sperm to eggs, by investigating the interaction of a number of polysaccharides and glycoconjugates with isolated bindin. Several of these polysaccharides inhibit the agglutination of eggs by bindin particles. An egg surface polysaccharide was found to be the most potent inhibitor ...

background: in this study the haemolymph components of infected and none infected lymnaea gedrosiana with xiphidiocercaria larvae was compared. methods: five hundred fifty lymnaea snails were collected from ilam and mazandaran prov­inces, iran, during 2008-2009. the snails were transported to the lab at tehran university of medi­cal sciences and their cercarial sheddings were studied. haemolmyp...

A unique sialic acid-binding lectin, achatininH (ATNH) was purified in single step from the haemolymph of the snail Achatina fulica by affinity chromatography on sheep submaxillary-gland mucin coupled to Sepharose 4B. The homogeneity was checked by alkaline gel electrophoresis, immunodiffusion and immunoelectrophoresis. Amino acid analysis showed that the lectin has a fairly high content of aci...

background: ‍‍cryopreservation has some detrimental impacts on sperms surface molecules. modification of the sperm surface molecules can affect on fertility rate. one of the important surface molecules are glycoconjugates. objective: the objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure. materials and methods: forty five h...

BACKGROUND In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared. METHODS Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran provinces, Iran, during 2008-2009. The snails were transported to the lab at Tehran University of Medical Sciences and their cercarial sheddings were studied. Haemolmyphs...

A lectin isolated from Rana catesbeiana eggs preferentially aggluti nates a large variety of human and animal tumor cells but not normal red blood cells, lymphocytes, or fibroblasts. The phenomenon correlates with a higher binding activity of the lectin with tumor cells. Chemical and physical analysis of the purified lectin indicates that the lectin is a low molecular weight basic polypeptide w...

Lectins are capable of cell agglutination by interacting with the surface exposed glycans. that agglutinate erythrocytes called hemagglutinin. These compounds occur in various organisms including plants. In this study, twenty edible vegetables were subjected to screen for haemagglutination and lectin activity. Lectin activity was assessed measuring inhibition ability different sugars. PBS (Phos...