Gloriosa superba L. a medicinally important monocot, member of the family Liliaceae, was investigated for callusing, direct regeneration and induction of hairy root using soil bacterium Agrobacterium rhizogenes. Friable callus was initiated from corms on basal MS medium amended with 2,4-D (1 mg L) and kinetin (1 mg L). Callus generated from such effective combinations were co-cultivated with th...

Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75% (w/v) glucose and at 22 degrees C. Dry sam...

Transgenic plants harboring the left transfer DNA (T(L)-DNA) of the root inducing plasmid of Agrobacterium rhizogenes show many developmental abnormalities. We observed frequent appearance of normal looking lateral (revertant) shoots from such aberrant plants. Unlike aberrant shoots of the plant, revertant shoots exhibited a very high growth rate and set viable seeds. Sexual and vegetative repr...

Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production and also influence numerous root developmental stages in largescale bioreactor culture. Due to the lack of biosynthetic nutrients and signalling-inducing nutrients, the regulatory role of mineral elements is not well understood. We have geneticall...

Functional analysis of promoter sequences is important to understand the regulation gene expression. This study aimed investigate region Lupinus luteus cytoplasmic cyclophilin (LlCyP; AF178458). After bioinformatic analysis, four deletion fragments were fused β-glucuronidase reporter gene. We used Lotus japonicus as a model plant. Agrobacterium rhizogenes transformation L. japonicus, only longe...