Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a...

Similar to Agrobacterium tumerfaciens, Agrobacterium rhizogenes can transfer foreign DNAs into plant cells based on the autonomous root-inducing (Ri) plasmid. A. rhizogenes can cause hairy root formation on plant tissues and form composite plants after transformation. On these composite plants, some of the regenerated roots are transgenic, carrying the wild type T-DNA and the engineered binary ...

Background: Common bean (Phaseolus vulgaris L.) is a globally important grain and vegetable legume crop, providing substantial portion of the diet protein minerals for many people in developing world. However, genetic studies improvement on this crop has long been impeded by its recalcitrance to Agrobacterium-mediated whole plant transformation. Established Agrobacterium rhizogenes-based hairy ...

The nutritional requirements of the tumor-inducing phytopathogenic bacteria and soil saprophytes which Conn (1942) has placed in the genus Agrobacterium have received somewhat more attention than have the requirements of most of the other groups of plant-disease bacteria. It is clear from the literature (Sagen, Riker, and Baldwin, 1934; Riker, Lyneis, and Locke, 1941; Hofer, 1941) that the crow...

The phylogenetic relatedness among 12 agriculturally important species in the order Rhizobiales was estimated by comparative 16S rRNA and dnaK sequence analyses. Two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. One group consisted of Mesorhizobium loti and Mesorhizobium ciceri, and the other group consisted of Agrobacterium rhizogenes, Rhizobium ...

Gesellschaft für Biotechnologische Forschung, Mascheroder Weg, D-3300 Braunschweig-Stöckheim, Bundesrepublik Deutschland Z. Naturforsch. 42c, 69—72 (1987); received August 21/September 22, 1986 Agrobacterium rhizogenes, Alkaloids, Nicotine, Cytisine, Nicotiana, Spartium The growth of root hairs from stems of Nicotiana tabacum and Spartium junceum was induced by Agrobacterium rhizogenes. Stem ro...

silymarin production by hairy root culture of milk thistle (silybum marianum l. gaertn) was investigated using agrobacterium rhizogenes ar15834. hairy roots were induced by injection or inoculation of explants with a. rhizogenes. one month old hairy roots were dissected from the explants and grown in murashing and skoog (ms) liquid medium. polymerase chain reaction (pcr) using the b gene and th...

A total of 57 colonies of Agrobacterium species was isolated from the root nodules of five different leguminous plant namely Pisum sativum, Sesbania rostrata, Vigna mungo, V. radita and V. ungiculata by using yeast extract mannitol agar (YEMA). All the isolated colonies were belonged to Agrobacterium species. The isolated bacteria were characterized and identified as Agrobacterium rhizogenes ba...

an efficient transformation system for the medicinal plant portulaca oleracea was established using agropine-type agrobacterium rhizogenes atcc15834. hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. the highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. roots grew...