Background: Gibberellic acid (GBA) is a naturally occurring and commercially produced plant growth regulator which may cause a variety of effects including the stimulation of seed germination in some cases. Histochemical changes in testicular tissue including carbohydrates, lipids and alkaline phosphatase enzyme changes were evaluated. Materials and Methods: Twenty seven mature male rats after ...

Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The ...

Since serum alkaline phosphatase increases in fluoride therapy for osteoporosis, it is generally accepted that fluoride stimulates bone formation. However, histochemical studies have shown that alkaline phosphatase is also increased in resorbing osteocytes. Fluoride is toxic to metabolically active bone cells, alkaline phosphatase is released, and serum alkaline phosphatase increases. We propos...

In a previous communication (Li, H.-C., Hsiao, K.-J., and Chan, W. W. S. (1978) Eur. J. Biochem 84,215-225) we reported the purification of a divalent cation-independent, nonspecific phosphoprotein phosphatase (phosphatase S, M, = 35,000) from canine cardiac muscle to apparent homogeneity. It was found that the homogeneous enzyme preparation exhibited significant activity toward p-nitrophenyl p...

Purified chicken intestinal alkaline phosphatase is active at pH 8 to 9, but becomes rapidly inactivated with change of pH to 6 or less. Also, a solution of the inactivated enzyme at pH 4.5 rapidly regains its activity at pH 8. In the range of pH 6 to 8 a solution of purified alkaline phosphatase consists of a mixture of active and inactive enzyme in equilibrium with each other. The rate of ina...

inor i8obutanol. Removal of the lipid is accompanied by simultaneous release of the enzyme into true solution. No other organic solvent tested was effective in releasing the bound phosphatase, indicating a unique action of butanol. I wish to thank Dr M. Dixon, F.R.S., for his encouraging interest and advice during this work. This work was carried out while holding a Travelling Scholarship award...

Phosphorylation of rabbit skeletal muscle glycogen synthase by cyclic AMP-independent synthase kinase 1 results in the incorporation of 4 mol of PO4/subunit. Incubation of the phosphorylated synthase with rabbit muscle phosphoprotein phosphatase brings about the hydrolysis of phosphates from all four major tryptic peptides and an increase in the synthase activity ratio from 0.01 to 0.85. Incuba...

Plasma alkaline phosphatase activity was measured in 349 infants aged between 5 and 10 days to establish a normal range for different gestational ages. Significant differences were observed between term and preterm infants, the highest values being associated with the shortest lengths of gestation. Plasma calcium, phosphate, and alkaline phosphatase activity were measured sequentially in 51 pre...

This study was aimed to determine the alkaline phosphatase activity following time course (days) in the intestine of broilers. We used a sample of 54 subjects of broilers from hatching period to 56th day of age. Our finding showed a significant increase in the alkaline phosphatase activity at various levels in the different intestinal segments during the first two weeks of life and then decreas...

An isolated marked transient rise in serum alkaline phosphatase levels in otherwise healthy children is a well-documented occurrence. However, in children undergoing liver transplantation, elevated alkaline phosphatase values raise the possibility of biliary obstruction, rejection, or both. During a 6-year period, 6 of 278 children undergoing liver transplantation exhibited a similar phenomenon...