Pseudomonas aeruginosa DQ8, which was isolated from the crude oil polluted soil in the Daqing oilfield of China, can efficiently degrade diesel, crude oil, n-alkanes, and polycyclic aromatic hydrocarbons (PAHs). Here, we present a 6.8-Mb assembly of its genome sequence. We have annotated 23 coding sequences (CDSs) responsible for catabolism of n-alkanes and PAHs.

We report the first extensive study of the spatial distribution and source apportionment of aliphatic hydrocarbons in soils from the Beijing outskirts. Mixed soil samples (5–30 cm depth) were collected for the quantitative determination of n-alkanes, isoprenoids, unresolved complex mixtures and the qualitative determination of hopanes and steranes. The total concentrations, including n-alkanes ...

Many biological and geochemical questions remain concerning the structures, functions, and properties of naturally occurring high-molecular-weight (C40+) alkanes with various mid-chain alkylation patterns. Above C40, these alkanes are exceedingly difficult to separate and purify, and syntheses can be blocked by the low solubility of intermediates. To overcome these problems, a facile three-step...

A method was developed to calculate the second dimension retention index of comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC/TOF-MS) data using n-alkanes as reference compounds. The retention times of the C(7)-C(31) alkanes acquired during 24 isothermal experiments cover the 0-6s retention time area in the second dimension retention time space, which make...

Twelve surface sediments from the southern Okinawa Trough (OT) and nine surface sediments from a nearby river, the Lanyang River (LR), with high sediment discharge were analyzed for comparison of their aliphatic hydrocarbon distributions. Performing cluster analysis on all hydrocarbon data of LR and OT sediments showed that the two areas had a similarity level of only 0.15, meaning that they we...

Effective application of whole-cell devices in synthetic biology and biocatalysis will always require consideration of the uptake of molecules of interest into the cell. Here we demonstrate that the AlkL protein from Pseudomonas putida GPo1 is an alkane import protein capable of industrially relevant rates of uptake of C7-C16 n-alkanes. Without alkL expression, native E.coli n-alkane uptake was...

in this work, we consider each normal alkane as a hypothetical mixtureof methyland methylene groups, in which the interaction potential of each pair is assumed to be the average effective pair potential. then, the lir equation of state (eos) is extended for such a hypothetical mixture. also, three basic compounds, namely, propane, n-butane and cyclohexane, are used to obtain the contribution of...

An alkane-degrading, sulfate-reducing bacterial strain, AK-01, isolated from a petroleum-contaminated sediment was studied to elucidate its mechanism of alkane metabolism. Total cellular fatty acids of AK-01 were predominantly C even when it was grown on C-even alkanes and were predominantly C odd when grown on C-odd alkanes, suggesting that the bacterium anaerobically oxidizes alkanes to fatty...

One of the difficulties in reporting accurate radiocarbon results from compoundspecific radiocarbon analysis (CSRA) is the lack of suitable process standard materials to correct for the amount and C content of carbon added during extensive sample processing. We evaluated the use of n-alkanes extracted from modern grass material (1.224 ±0.006 fraction modern) as process standards for CSRA. The n...

We studied the effect of segmented solvent molecules on the free energy of transfer of small molecules from water into alkanes (hexane, heptane, octane, decane, dodecane, tetradecane, and hexadecane). For these alkanes we measured partition coefficients of benzene, 3-methylindole (3MI), 2,3,4,6-tetrachlorophenol (TeCP), and 2,4,6-tribromophenol (TriBP) at 3, 11, 20, 33 [corrected], and 47 degre...