We have previously isolated mutants of Escherichia coli that replicate their DNA with increased fidelity. These mutants have a mutation in the dnaE gene, encoding the a subunit of DNA polymerase 111. They were isolated in a mismatch-repairdefective mutL background, in which mutations can be considered to represent uncorrected DNA replication errors. In the present study we analyze the effect of...

The age of an allele can be estimated both from genetic variation among different copies (intra-allelic variation) and from its frequency. Estimates based on intra-allelic variation follow from the exponential decay of linkage disequilibrium because of recombination and mutation. The confidence interval depends both on the uncertainty in recombination and mutation rates and on randomness of the...

The interest in human genetic diversity for understanding disease etiology, toxicology, and response to therapy has grown in recent years into an active field of research. For example, in cancer epidemiology, and especially in the field of molecular epidemiology, a great deal of attention has been paid to the role of common population polymorphisms in genes controlling carcinogen metabolism. Th...

The objective of this study was to determine the polymorphism of bovine β-lactoglobulin (β-LG) gene and its association with milk production traits in Iranian Najdi cattle. Blood samples were collected from 80 Najdi cattle from the Najdi cattle breeding station located in Shoshtar, Khuzestan. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniqus were used...

The new CFTR modulator combination, elexacaftor/tezacaftor/ivacaftor (Trikafta) was approved by the FDA in October 2019 for treatment of Cystic Fibrosis patients 6 years age or older who have at least one F508del mutation allele and a minimal-function another other allele. However, there is group patients, addition to those with rare mutations, which despite presence allele, it not possible ide...

Abstract Genotyping individuals using forensic or non-invasive samples such as hair fecal increases the risk of allelic amplification failure (dropout) due to low quality and quantity DNA. One way decrease genotyping errors is increase number replicates per sample. Here, we have developed software SNP+ estimate dropout probability subsequent required obtain reliable genotype with 95%. Moreover,...

multiple sclerosis (ms) is a chronic inflammatory disorder of the central nervous system, with a complex etiology that includes a strong genetic component. the chromosome 19q 13 region surrounding the apolipoprotein e (apoe) gene has shown consistent evidence of involvement in ms. in a cross-sectional study, to show the apoe genotype and allele frequency in the ms population of iran in comparis...

The aim of the present study was the conducting of comparative analysis of allele pool of Ukrainian population of Thoroughbred horses and the populations from England, USA, Russia and South Korea using microsatellite loci of DNA on the basis of our own researches and literary sources. Comparative analysis of allele pool of Thoroughbred populations from different countries was conducted using 6 ...

The GROWTH-REGULATING FACTOR4 (OsGRF4) allele is an important target for the development of new high nitrogen-use efficiency (NUE) rice lines that would require less fertilizers. Detection OsGRF4 through PCR (polymerase chain reaction)-based assay cumbersome and needs advanced laboratory skills facilities. Hence, a method conveniently rapidly detecting on-field key requirement further research ...