Source and Description of Sequence Polymorphism: Nucleotide sequencing of type HI procollagen (COL3A1) cDNAs from several individuals revealed a nucleotide polymorphism that converted the codon for amino acid 581 of the triple helix from -GGC(C-allele) (1) to -GGT(T-allele) in some alleles. The PCR was carried out with primers below; cDNA products were 226 bp and genomic products were 326 bp in...

Reported estimates of ras mutation prevalence in lung adenocarcinoma of 15-24% may be underestimates because of the insensitivity of the assays used. We have devised a rapid, non-radioactive assay for ras mutations, which detects 1 mutant allele/10(3) normal alleles and have used it to study DNA isolated from 53 lung tumor samples (including 28 adenocarcinomas) previously analyzed by PCR/allele...

This report details the precise mapping of a partially deleted human antithrombin III (AT-III) allele, found in a kindred with an inherited type 1 AT-III deficiency. Using truncated AT-III probes generated by polymerase chain reaction (PCR) amplification from a full-length AT-III cDNA, as well as other genomic probes specific for the 5' upstream region of the AT-III gene, we were able to charac...

Allele-specific amplification on the basis of polymerase chain reaction (PCR) has been widely used for single-nucleotide polymorphism (SNP) genotyping. However, the extraction of PCR-compatible genomic DNA from whole blood is usually required. This process is complicated and tedious, and is prone to cause cross-contamination between samples. To facilitate direct PCR amplification from whole blo...

INTRODUCTION: Anaplastic thyroid carcinoma (ATC) is the most aggressive type of cancer accounting for 12% all malignancies. Systemic therapy remains main treatment strategy. Targeted and immunotherapy are prescribed when certain molecular genetic aberrations detected.
 THE AIM: To investigate profile samples anaplastic carcinoma.
 MATERIALS AND METHODS: The study included 37 patients ...

ABSTRACT A multiplex real-time polymerase chain reaction (PCR) assay was developed to simultaneously genotype plants for the I and bc-1(2) alleles, which condition resistance in beans to Bean common mosaic virus and Bean common mosaic necrosis virus. A segregating F(2) population was derived from the cross between pinto bean breeding line P94207-189A (bc-1 bc-1 I I) and Olathe (bc-12 bc-1(2) i ...

Panax ginseng is one of the most valuable medicinal plants in the Orient. The low level of genetic variation has limited the application of molecular markers for cultivar authentication and marker-assisted selection in cultivated ginseng. To exploit DNA polymorphism within ginseng cultivars, ginseng expressed sequence tags (ESTs) were searched against the potential intron polymorphism (PIP) dat...