chemokine and chemokine receptors show several variations which may affect resistance to infectious disease. a 32 base pair deletion in the open reading frame of the human ccr5 gene (ccr5?32) results in producing a truncated antigen which fails to be presented on the surface of target cells. ccr5?32 variant is not a functional co receptor for hiv-1 entrance and delay the onset of acquired immun...

Abstract Background The prevalence of gastrointestinal (GI) neoplastic polyps in Jack Russell terriers (JRTs) has increased Japan since the late 2000s. Recently, we demonstrated that JRTs with GI harbor identical germline variant APC gene (c.[462_463delinsTT]) heterozygous state. Thus, this disease is an autosomal dominant hereditary disorder. Although affected have distinct features, such as d...

The ease and advantages of allelespecific PCR (AS-PCR) for SNP genotyping, along with the difficulty of assay specificity with certain mismatched DNA primers, have been described in prior studies (1–3). More recently, the use of real-time PCR detection formats has been described for one or both alleles of AS-PCR (4–6). These detection enhancement methods do not overcome the inherent difficultie...

Despite significant advances in understanding the molecular basis of sepsis and its associated immunological response, sepsis remains a problem worldwide and is associated with a high mortality. Annually, septic shock, the most severe form of sepsis, causes the death of more than 100 000 people in the USA (3,4). Gram-negative bacteria are the most common pathogens associated with bacterial infe...

The IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.