Ferrite grade zinc oxide was prepared through aqueous processing of zinc ash followed by calcination. Zinc was quantitatively dissolved in sulphuric acid medium. The ferrous iron present in the leach solution was oxidised with hydrogen peroxide followed by precipitation of total iron by pH adjustment to 4.5 with dilute sodium hydroxide solution. Copper was removed by cementation using zinc dust...

HBs vaccines were prepared by three different methods: 1) Heat inactivation (Krugman, et.al 1971)2) Ammonium sulphate precipitation followed by column chromatography (Sephadex G-200) 3) PEG-6000 (polyethylene glycol) precipitation followed by column chromatography using Sephadex G-200. Their efficacy was studied in guinea pigs, and the results compared with commercial Japanese vaccine (Gr...

The pH-dependence of sulphate-activated phosphorylase b has been studied in the direction of glycogen synthesis. The bell-shaped curve of the pH-dependence of the catalytic constant for the AMP-activated enzyme showed pK values of 6.1 and 7.3, but the curve for the enzyme activated by 0.9 M ammonium sulphate showed a drop of activity on the acid side at much higher pH values. Its bell was centr...

A simple spectrophotometric method is reported her for the estimation of reserpine in polyherbal formulations. Estimation is based on the reaction with3-methylbenzolinone-2hydrazone (MBTH) reagent in presence of cerric ammonium sulphate to yield a violet coloured chromogen, which exhibits and absorption maxima at 580 nm. The chromogen is stable for 10 minutes.

Nitrite oxidase and nitrate reductase in Nitrobacter agilis were shown to be separate enzymes. The best separation of the two systems was achieved by ammonium sulphate fractionation. The effects of various compounds, including antimycin A, 2-n-heptyl-4-hydroxyquinoline N-oxide and chlorate, also clearly distinguish between the two enzyme reactions. The relationship between the two opposing reac...

Studies of alpha-amylase production by Bacillus subtilis (CM3) isolated earlier from cow dung microflora, were carried out. The optimum temperature, pH and incubation period for amylase production were 50-70 degrees C, 5.0-9.0 and 36 h, respectively. Enzyme secretion was very similar in the presence of any of the carbon sources tested (soluble starch, potato starch, cassava starch, wheat flour,...

Although laboratory studies show that biogenic volatile organic compounds (VOCs) yield substantial secondary organic aerosol (SOA), production of biogenic SOA as indicated by upward fluxes has not been conclusively observed over forests. Further, while aerosols are known to deposit to surfaces, few techniques exist to provide chemically-resolved particle deposition fluxes. To better constrain a...

Human milk was fractionated by ammonium sulphate precipitation and column chromatography. A milk fraction depleted of secretory immunoglobulin A and with an apparent molecular weight of greater than 400,000 inhibited fluid secretion induced by cholera toxin and Escherichia coli heat-labile toxin in rabbit ileal loops.

This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.

1. alpha-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.