Amylase activity and mRNA abundance are reduced to less than 1% of normal levels in diabetic rat pancreas. Administration of vanadate in drinking water restored normal amylase activity and amylase mRNA levels. These results demonstrate an effect of vanadate on pancreatic acinar cells and suggest that vanadate can mimic the effect of insulin on transcription of the pancreatic amylase gene.

Drying of seeds of Agropyron desertorum (Fisch. ex Link) Schult. did not result in breakdown of alpha-amylase nor impair the ability of seeds to resume its synthesis when moistened again. beta-Amylase activity did not change during 5 days of germination at a water potential of 0 atmosphere nor during 40 days of incubation at -40 atmospheres. Seeds synthesized alpha-amylase at 0, -20, and -40 at...

-Different amylase enzymes were identified by analysis of digestive fluid and haemolymph in diapausing and nondiapausing strains of silkworm, Bombyx mori. The diapausing strain showed negligible digestive amylase activity at a pH range of 3-11, while the nondiapausing strain registered strikingly higher amylase activity at pH 9.2. Higher levels of undigested starch was found in the faecal matte...

Clostridium thermosulfurogenes, an anaerobic bacterium which ferments starch into ethanol at 62 degrees C, produced an active extracellular amylase and contained intracellular glucoamylase but not pullulanase activity. The extracellular amylase was purified 2.4-fold, and its general physicochemical and catalytic properties were examined. The extracellular amylase was characterized as a beta-amy...

Midgut α-amylase is an important digestive enzyme involved in larval energy metabolism and carbohydrate assimilation. In this article, the properties of midgut α-amylase from the Oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae), larvae were characterized, and its in vitro responses to chemical inhibitors were also determined. The kinetic parameters Km and Vmax of midgut α-amylase w...

There have been several indications (1, 2) that the amylase found in serum is derived from sources other than the pancreas or salivary glands. For this reason we wished to determine whether any indication of fractionation of serum amylase could be obtained by paper electrophoresis. Although several accounts of the fractionation of amylase in serum are found in the literature, some involving pre...

The aim of this study was production and partial purification of α-amylase enzyme by Bacillus licheniformis. B. Licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. Optimal conditions for amylase production by B. Licheniformis are incubation period of 120 h, temperature of 37 °C and pH 7.0. The α-amylase enzyme was purified by ion exchange chromatography ...

In maltose syrup production, one of the critical processing stages is starch conversion process. During this process, reaction time and enzyme concentrations are two important parameters to obtain standard sugar spectrum. The purpose study is; i) find optimum during ii) determine process control dynamic in production. different amounts beta alpha-amylase enzymes (0.10, 0.15, 0.20 0.25 ml β-amyl...

Analysis of the structures of the human amylase genes has demonstrated that this multigene family contains at least five tandem gene copies, closely related in sequence but with distinct tissue specific expression. The structures of the genes demonstrate that the human salivary amylase gene was derived from a preexisting pancreatic amylase gene. Insertion of a retrovirus upstream of the amylase...

Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas(®) Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5-32 μL) with high or intermediate ...