background: the present study has been carried out to investigate the effects of preovulatory stage gamma-irradiation of female mice in the absence or presence of vitamin e on numerical chromosome abnormalities in 8-cell embryos after mating with non-irradiated males. materials and methods: the 8-11 weeks adult female nmri mice were whole body irradiated at preovulatory stage (post pmsg injecti...

objective: to study the structure and distribution of microtubules in embryos derived from young, old and reconstructed oocytes. materials and methods: embryos obtained from old (50 embryos), young (50 embryos) and reconstructed oocytes (10 embryos) were studied by immunocytochemistry. the microtubule structures of the embryos were studied by using fluroscent microscopy with fitc-pi filter and ...

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...

INTRODUCTION Tuberculous pleural effusions are not always easy to diagnose but the presence of a lymphocyte-rich exudate associated with an increased adenosine deaminase level and a positive skin test result are highly sensitive diagnostic signs. CASE PRESENTATION We report a case of pleural tuberculosis in a 31-year-old white male patient from Caracas, Venezuela who was negative for human im...

The marked sexual dimorphism that exists in human cardiovascular diseases has led to the dogmatic concept that testosterone (Tes) has deleterious effects and exacerbates the development of cardiovascular disease in males. While some animal studies suggest that Tes does exert deleterious effects by enhancing vascular tone through acute or chronic mechanisms, accumulating evidence suggests that T...

Little is known concerning the potential ecological effects of hormonally active substances associated with discharges from animal feeding operations. Trenbolone acetate is a synthetic anabolic steroid that is widely used in the United States to promote growth of beef cattle. Metabolites of trenbolone acetate include the stereoisomers 17alpha- and 17beta-trenbolone, both of which are stable in ...

introduction: this study was designed to compare the effects of three vitrification procedures [conventional (c), open pulled straw (ops) and closed pulled straw (cps)] on morphological survival of two-cell mouse embryos and their subsequent development to hatched blastocysts. material and methods: two-cell mouse embryos were divided into four groups, control (194), ops (150), cps (155) and c (...

introduction: the incidence of chromosomal abnormalities was investigated in untransfered embryos reuslted from in vitro fertilization (ivf) or intracytoplasmic sperm injection (icsi) procedures. materials and methods: a total of 238 embryos of varying morphology between the pronucleated stage and 8-cells were analysed. the cytogenetic method of dyban was used for chromosome preparation. embryo...

introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...