The numbers of antiplasmin, antitrypsin and antichymotrypsin in human plasma and mutual relationship among them are discussed in this paper. Human blood plasma was divided into 2 major fractions by adding ammo nium sulfate, after desalting by sephadex, the fractions being further subfrac tionated by using column chromatographic procedure on DEAE-cellulose. The determination of antiproteolytic a...

HLA-A, B and alpha 1-antitrypsin phenotypes were studied in 90 unrelated patients with established nodal generalised osteoarthritis (OA). Compared with standard reference populations, independently increased frequency of the HLA-A1B8 and MZ alpha 1-antitrypsin phenotypes were observed (27% v 11.5%, relative risk 2.79, and 12% v 3.6%, relative risk 3.73 respectively). These associations related ...

Overexpression of Z α1-antitrypsin is known to induce polymer formation, prime the cells for endoplasmic reticulum stress and initiate nuclear factor kappa B (NF-κB) signalling. However, whether endogenous expression in primary bronchial epithelial cells has similar consequences remains unclear. Moreover, the mechanism of NF-κB activation has not yet been elucidated. Here, we report excessive N...

We present a new, simple, and inexpensive sandwichtype double-antibody enzyme immunoassay for alpha,antitrypsin in dried blood collected on the fifth day postpartum. The method is very sensitive, having a detection limit of 2.84 fmol/well. Intraand interassay CVs are 6.1% and 10.3%, respectively, for assay of 5-mm-diameter blood spots eluted into 7 mL of phosphate buffer. Since February 1984, w...

A case of immunoglobulin G (kappa) myeloma showed, in addition to the monoclonal IgG(kappa) arc, two kappa chains in the serum. The urine specimen contained 7.75 g of kappa chains per liter. The electrophoretically fast-moving kappa chain in serum was shown by immunoelectrophoresis and Ouchterlony immunodiffusion to be a complex of kappa chains and alpha 1-antitrypsin. This complex, which was d...

Alpha-1 antitrypsin deficiency is an inherited disorder that may cause severe lung and liver disease.

Inhibitors of fibrinolysis may be divided into naturally circulating inhibitors and antifibrinolytic agents. Natural inhibitors are present in plasma and are components of blood cells, especially platelets. Several types of antifibrinolytic activity can be distinguished: antiplasmin activity, and anti-activator activity against streptokinase, urokinase, and tissue activator induced plasminogen ...

Whether genetic factors other than the protease-inhibitor (PI) polymorphism itself contribute to variation in alpha-1-antitrypsin is of both theoretical and practical interest. We have measured the quantity of alpha-1-antitrypsin (by an immunoturbidometric assay) and its activity (by assaying elastase inhibitory capacity [EIC]) in 583 individuals from 114 twin kinships who were also typed for P...

OBJECTIVE The formation of neutrophil extracellular traps and the exposure of nucleosomes on these neutrophil extracellular traps contribute to coagulation activation and the propagation of deep vein thrombosis (DVT) in animal models. However, no data are available on the role of neutrophil extracellular traps or nucleosomes in patients with thrombosis. METHODS AND RESULTS We conducted a case...