This study evaluated the influence of apical patency, root filling removal technique and cleaning of the apical foramen, concerning the amount of debris extruded during root canal retreatment. Forty mandibular incisors were randomly assigned to 4 groups - GIM (n=10), GIIM (n=10), GIPT (n=10) and GIIPT (n=10), which were named according to leaving (I) or not (II) apical patency during canal prep...

The objective was to compare the role of needles design (open ended versus closed ended) on the apical extrusion of sodium hypochlorite irrigant during root canal treatment. This was an in vitro study performed at the Department of Operative Dentistry, Liaquat University of Medical & Health Sciences, Jamshoro from March 2011 to October 2011. Access cavities were prepared in one hundred extracte...

The essential steps for successful endodontic treatment consist of elimination of all diseased pulp and dentin tissues, adequate chemomechanical preparation and filling of the root canal system hermetically.[1] Moreover, it is crucial that root canal obturation has to be limited within root canal system. Minor apical foramen or minor apical constriction is known to be situated 0.5–1.5 mm corona...

During cruciform extrusion, a DNA inverted repeat unwinds and forms a four-way junction in which two of the branches consist of hairpin structures obtained by self-pairing of the inverted repeats. Here, we use single-molecule DNA nanomanipulation to monitor in real-time cruciform extrusion and rewinding. This allows us to determine the size of the cruciform to nearly base pair accuracy and its ...

Despite high rates of cell death, epithelia maintain intact barriers by squeezing dying cells out using a process termed cell extrusion. Cells can extrude apically into the lumen or basally into the tissue the epithelium encases, depending on whether actin and myosin contract at the cell base or apex, respectively. We previously found that microtubules in cells surrounding a dying cell target p...

Besides the risk of filling material extrusion throughout the apex, a satisfactory apical seal can be difficult to achieve in canals with open apices or iatrogenic enlargements of the apical constriction. These situations pose a challenge to root canal filling. This paper describes the root canal filling of a maxillary right canine with an overinstrumented apex, complete loss of the apical stop...