Cerebellar neurons, cultured on monolayers of 3T3 fibroblasts or on a polylysine/laminin-coated substratum, responded to recombinant basic FGF by extending longer neurites. The response was biphasic reaching a maximum at 5 ng/ml FGF, but desensitising at 100-200 ng/ml FGF. The response to FGF could be inhibited by a tyrosine kinase inhibitor (the erbstatin analogue), by a diacylglycerol lipase ...

Rabbit neutrophils were stimulated with the chemotactic peptide fMet-Leu-Phe in the presence of the methyltransferase inhibitors homocysteine (HCYS) and 3-deazaadenosine (3-DZA). HCYS and 3-DZA inhibited chemotaxis, phospholipid methylation, and protein carboxymethylation in a dose-dependent manner. The chemotactic peptide-stimulated release of [14C]arachidonic acid previously incorporated into...

Arachidonic acid (250 micrograms) injected into the left circumflex coronary artery of anesthetized dogs, with carotid arteries tied to reduce baroreflex-induced changes, caused a reduction in heart rate (-15 +/- 1.6 beats/min), mean arterial pressure (-27 +/- 3.4 mm Hg), and left ventricular dP/dt (-939 +/- 138 mm Hg/sec). In contrast, injection of arachidonic acid into the left anterior desce...

Cancer and inflammation are intimately linked due to specific oxidative processes in the tumor microenvironment. Lipoxygenases are a versatile class of oxidative enzymes involved in arachidonic acid metabolism. An increasing number of arachidonic acid metabolites is being discovered and apart from their classically recognized pro-inflammatory effects, anti-inflammatory effects are also being de...

Arachidonic acid at micromolar concentrations produced a drastic increase of the generation of reactive oxygen species (ROS) in rat hepatoma AS-30D cells cultivated in vitro along with an increase in the incidence of apoptotic cell death. Both processes were prevented by trolox, a water-soluble tocopherol derivative, and tempol, a known antioxidative agent. A synthetic hybrid of lipoic acid and...

The synthesis of prostaglandins F2alpha and E2 by guinea-pig uterine homogenates was inhibited by p-bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A2. 2 Metabolism of prostaglandin F2alpha by uterine homogenates was undetectable; this was not affected by PBPAB. 3 There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7...

Abstract Patients undergoing cardiac surgery are at risk of excessive bleeding and its associated complications. Excessive during after has an incidence ~20%. Massive subsequent requirement for blood product administration mediastinal reexploration with significant morbidity mortality. Postoperative, nonsurgical in surgical patients is often multifactorial. Platelet dysfunction, fibrinolysis, h...

Abrtract The ethanolamine-containing glycerophospholipids, choline-containing glycerophospholipids, and phosphatidylinmitol fractions are major sources of arachidonic acid in murine mastocytoma P-815 cloned cells. The choline-linked fraction contained high arachidonic acid contents in 1-0-alkyl-2-acyl(18%) and 1,2-diacyl-sn-glycero-3-phosphocholine (ll%), with smaller amounts in l-O-alk-l'-enyl...

Brain astrocytes in primary culture from the rat or the mouse have been shown to possess ionotropic and metabotropic glutamatergic receptors. The activation of both types of receptors is responsible for a rise in the cytosolic concentration of calcium, while the stimulation of metabotropic receptors induces the accumulation of inositol phosphates. In the present study, it is demonstrated that i...

Arachidonic acid, a precursor of prostaglandin E!} administered topically to rabbit and monkey eyes in concentrations of 0.2 to 20 per cent, or intravenously to rabbits in doses of 10 to 20 mg., produced a significant elevation of intraocular pressure. A dose-response relationship was demonstrated. Facility of outflow and anterior chamber aqueous protein were significantly higher in eyes treate...