Abstract Background The prevalence and genotypes of G6PD deficiency vary worldwide, with higher in malaria endemic areas. first-time assessment molecular characterization mutations the Lao Theung population were performed this study. Methods A total 252 unrelated participants residing People’s Democratic Republic (PDR) recruited. All participant samples tested for enzyme activity gene mutations...

Waxy sorghum has greater economic value than wild in relation to their use food processing and the brewing industry. Thus, authentication of waxy species is an important issue. Herein, a rapid sensitive Authentication Amplification Refractory Mutation System-PCR (aARMS-PCR) method was employed identify via its ability resolve single-nucleotide genes. As proof concept, we chose containing wxc mu...

BACKGROUND Oxaliplatin-capecitabine (OxCap) and carboplatin-paclitaxel (CarPac) based neo-adjuvant chemoradiotherapy (nCRT) have shown promising activity in localised, resectable oesophageal cancer. PATIENTS AND METHODS A non-blinded, randomised (1:1 via a centralised computer system), 'pick a winner' phase II trial. Patients with resectable oesophageal adenocarcinoma ≥ cT3 and/or ≥ cN1 were ...

PURPOSE Epidermal growth factor receptor (EGFR) mutations are prerequisites for the targeted therapy with anti-EGFR tyrosine kinase inhibitors (TKIs) in non-small-cell lung carcinomas (NSCLCs). In patients with advanced-stage NSCLC, sometimes cytological specimens, including those from fine-needle aspiration cytology (FNAC) and pleural effusion, are the only materials for mutation analysis. The...

BACKGROUND Warfarin is the most commonly prescribed oral anticoagulant medication but also is the second leading cause of emergency room visits for adverse drug reactions. Genetic testing for warfarin sensitivity may reduce hospitalization rates, but prospective genotyping is impeded in part by the turnaround time and costs of genotyping. Microfluidics-based assays can reduce reagent consumptio...

Polymerase chain reaction (PCR) is a powerful molecular biological tool in the field of toxicity testing and diagnostics. The use of PCR for large-scale genetic testing requires an effective method of sample processing. Unfortunately, isolation of PCR-quality DNA is time-consuming. PCR performed directly on whole blood is preferred because of time efficiency, cost of the procedure, and possible...