A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.

*Corresponding author: Dominika Szőke, UOC Patologia Clinica, Azienda Ospedaliera “Luigi Sacco”, Via GB Grassi 74, 20157 Milan, Italy, Phone: +39 02 39042683, Fax: +39 02 39042364, E-mail: [email protected]; and Clinical Pathology Unit, “Luigi Sacco” University Hospital, Milan, Italy Assunta Carnevale and Sara Pasqualetti: Clinical Pathology Unit, “Luigi Sacco” University Hospital, Milan...

A 2-site immunoradiometric assay (IRMA) was performed on the head and thorax of Anopheles culicifacies s.l. and An. pulcherrimus females, the 2 most common anopheline species in the District of Ghassreghand (Baluchistan, Iran), collected during the 2 peak malaria transmission seasons (May and September-October 1991). Positive IRMA results revealed the 2 species as potential vectors of malaria i...

A B S T R A C T Cell-mediated and humoral immilune responses of mutant diabetic db+/db+ mice were evaluated using in vivo and in vitro immunological assays. When compared to lean, nondiabetic db+/m+ or m+/m+ mice, db+/db+ mice demonstrated markedly altered in vivo immune responses characterized by a significantly diminished ability to reject allogeneic skin grafts, a markedly diminished capacit...

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide...