Phytochemical screening and antimicrobial activity of chloroform, acetone and methanolic extracts of Calotropis procera (Ait.) R. Br. (Asclepiadaceae) flowers were tested against various pathogens such as Salmonella paratyphi, S. paratyphi A, Bacillus subtilis, Bacillus thuringiensis, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. Phytoc...

A rapid and cost-effective colorimetric sensor has been developed for the detection of bacteria (Bacillus subtilis was selected as an example). The sensor was designed to rely on lysozyme-capped AuNPs with the advantages of effective amplification and high specificity. In the sensing system, lysozyme was able to bind strongly to Bacillus subtilis, which effectively induced a color change of the...

Bacillus thuringiensis produces crystal proteins (Cry) that account for up to 25% of the dry cell weight during the stationary phase. The high-level expression and stationary phase-specific autoinduction of the cry gene led to development of a cry promoter-based Bacillus expression system. Among the various cry promoters, cry3Aa promoter was selected by comparing the lacZ expression levels in B...

Microbial degradation of petroleum hydrocarbons is one of the major practices in natural decontamination process. The present study investigated about the isolation of bacteria from crude oil contaminated site and gravimetric analysis of degradation in which, two bacterial isolates formed maximum clearing zone on mineral salt medium. Among these isolate S2 showed maximum growth (0.85mg/ml) and ...

The beneficial effects of probiotics have been attributed to their ability to promote the immunological and nonimmunological defense barrier in the gut, normalization of increased intestinal permeability and altered gut microflora. Twelve different intestinal bacterial colonies were isolated from Black Tiger Shrimp (Penaeus monodon). Among them strain PPP 13 was studied and characterized due to...

Ten new azo Schiff bases 5a-h and 7a-b were prepared in excellent yields via the condensation of different aromatic amines and a new azoaldehyde, 2-hydroxy-3- methoxy-5-(4-methoxyphenylazo)benzaldehyde (4) by two different methods. All new compounds were tested against five microorganisms: Staphylococcus aureus (Gram positive and methicillin resistant), Bacillus subtilis (Gram positive), Kelebs...

A Bacillus subtilis strain was isolated from the intestine of Sebastiscus marmoratus (scorpion fish) that was identified as Bacillus subtilis CH2 by morphological, biochemical, and genetic analyses. The chitosanase of Bacillus subtilis CH2 was best induced by fructose and not induced with chitosan, unlike other chitosanases. The strain was incubated in LB broth, and the chitosanase secreted int...

Streptococcus cremoris Wg2 plasmid pWV01 was introduced in Bacillus subtilis by protoplast transformation. The yield of pWV01 isolated from B. subtilis was low. pWV01 contains a unique site for the restriction endonuclease MboI.

In Bacillus subtilis, an RNA binding protein called TRAP regulates both transcription and translation of the tryptophan biosynthetic genes. Bacillus halodurans is an alkaliphilic Bacillus species that grows at high pHs. Previous studies of this bacterium have focused on mechanisms of adaptation for growth in alkaline environments. We have characterized the regulation of the tryptophan biosynthe...

Earlier phylogenetic studies based on the inferred DNA sequences of the polC, rpoB and gyrA genes suggested that strains of the species Bacillus subtilis formed two clusters, indicating the presence two closely related taxa; one contained the laboratory strain 168 and the other the laboratory strain W23. Significant sexual isolation was found between strain 168 and members of the group containi...