Objective: Lactic acid bacteria (LAB) produce various substances that exhibit antagonistic activity against other bacteria. Detection of antimicrobial substance produced by lactic acid bacteria against the water borne pathogens is the objective of this work. Materials: The bacteriocin producing probiotic strain Lactobacillus plantarum was isolated from a grass silage samples and identified base...

The aim of this work was to isolate and characterize bacteriocins produced by 2 Lactobacillus fermentum strains isolated from artisanal Mexican Cocido cheese. Fractions (F ≤3 kDa) obtained cell-free supernatants Lb. J23 J32 were further fractionated reversed-phase HPLC on a C18 column. Antimicrobial activities F kDa bacteriocin-containing fractions (BCF), fractionation against 4 indicator micro...

Production of enterocin AS-48 by Enterococcus faecalis A-48-32 was compared between standard and high-cell density batch fermentations. In high-cell density cultures, bacteriocin production was 2.47-fold higher, provided that the pH was controlled during the fermentation. A two-step procedure for recovery of milligram quantities of purified bacteriocin was developed, based on adsorption of the ...

The objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption. The virulence and bacteriocin genes of 78 E. faecalis were amplified by PCR and visualized in agarose gels. The antimicrobial susceptibility was determined through diffusion agar te...

By conducting a systemic screening from Chinese traditional Tofu, a bacteriocin producer with broad antimicrobial spectrum was found. The producer was identified by 16S rDNA sequencing, and it was identified as Leuconostoc mesenteroides. The producer possessed typical bacteriocin traits, such as be heat-stable and proteinase-labile. Its inhibition spectrum contained many distantly related gener...

Bacteriocins are secreted oligopeptides, proteins or protein complexes with antimicrobial activity against strains taxonomically related to the producer organism (2, 10). In recent years, numerous reports have been published on antimicrobial peptides or proteins produced by gram-positive bacteria, including lactic acid bacteria (LAB) (4, 7), and bacteriocins for lactobacilli from different envi...

Abstract. Nasution SA, Fachrial E, Ginting CN, Liena, Piska F. 2023. Bacteriocin-producing Pediococcus acidilactici BAMA 15 isolated from "naniura" traditional foods in North Sumatra, Indonesia. Biodiversitas 24: 2830-2835. Lactic Acid Bacteria (LAB) have beneficial properties for their hosts, potentially promoting health and balancing the normal gut flora. Meanwhile, bacteriocin is a bacterios...

In this work, we report the expression and secretion of the leaderless two-peptide (EntL50A and EntL50B) bacteriocin enterocin L50 from Enterococcus faecium L50 by the methylotrophic yeast Pichia pastoris X-33. The bacteriocin structural genes entL50A and entL50B were fused to the Saccharomyces cerevisiae gene region encoding the mating pheromone alpha-factor 1 secretion signal (MFalpha1(s)) an...

Lactic acid bacteria (LAB) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (GRAS) by the FDA for use as food ingredients. In addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. In this study we examined the inhibition of Listeria monocytogenes 39-2 by neutralized and non-n...

Lacticin 3147 is a broad-spectrum bacteriocin produced by Lactococcus lactis subsp. lactis DPC3147 (M. P. Ryan, M. C. Rea, C. Hill, and R. P. Ross, Appl. Environ. Microbiol. 62:612-619, 1996). Partial purification of the bacteriocin by hydrophobic interaction chromatography and reverse-phase fast protein liquid chromatography revealed that two components are required for full activity. Lacticin...