Benzopyrene (BaP) is polycyclic aromatic hydrocarbon (PAH), and is chemically modified in the animal body to form a number of metabolites that may elicit a various toxicity. However, the effect of BaP on neuroblastoma differentiation has remained unclear. We have studied the effect of BaP on neurite outgrowth using human SH-SY5Y neuroblastoma cells induced to differentiate by all-trans-retinoic...

We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of receptors and the generation of monospecific antibodies (Rothenberg et al., Proc. Na...

Benzo(a)pyrene (BaP), a major environmental pollutant and component of cigarette smoke, is both carcinogenic and atherogenic in experimental models. We investigated the effect of long-term administration of BaP on atherogenesis in both atherosclerosis-susceptible White Carneau (WC) and atherosclerosis-resistant Show Racer (SR) pigeons. The number and size of arterial lesions in the brachiocepha...

OBJECTIVE To evaluate the possible vascular effects of an environment carcinogen benzo(a)pyrene (BaP). METHODS The cytotoxicit of BaP and rat liver S9 (0.25 mg/mL)-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10(-6) mol/L phenylephrine (PE) in an ex-vivo perfusion system aft...

The metabolism of benzo(a)pyrene (BaP) by hepatic or cheek pouch epithelium microsomes obtained from Syrian golden ham sters which had been consuming an ethanol-containing liquid diet for 4 wk and from pair-fed controls was measured. Gluta thione S-transferase activity with 1-chloro-2,4-dinitrobenzene or (±)-r-7,f-8-dihydroxy-f-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene as substrates was mea...

The pharmacokinetics of [(3)H]-9-hydroxybenzo[a]pyrene (9-OH-BaP), a highly lipophilic primary metabolite of benzo(a)pyrene, were examined after intrapericardial (iv) or oral doses of 50 or 200 microg/kg to intermolt American lobsters, Homarus americanus. Combining data for all lobsters, the average terminal elimination half-life of parent 9-OH-BaP was 97.3 h after iv and 56.5 h after oral admi...

UDP-glucuronosyltransferase 1A10 (UGT1A10) is an extrahepatic enzyme expressed in aerodigestive tract tissues that exhibits significant glucuronidation activity against the important procarcinogenic benzo(a)pyrene (BaP) metabolite, BaP-trans-7,8-dihydrodiol (BPD), and the UGT1A10 codon 139 (Glu>Lys) polymorphism was previously implicated in risk for orolaryngeal cancer by Elahi et al. in their ...

The absorption efficiency (AE) of benzo[a]pyrene (BaP), a representative polycyclic aromatic hydrocarbon compound, in the green mussel Perna viridis was measured under different biological and food conditions (body size, food quantity, and food species). Uptake of BaP from the aqueous phase by different species of phytoplankton and green mussels was also experimentally quantified. A bioenergeti...

The metabolism of benzo(a)pyrene (BaP) by hepatic or cheek pouch epithelium microsomes obtained from Syrian golden hamsters which had been consuming an ethanol-containing liquid diet for 4 wk and from pair-fed controls was measured. Glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene or (+/-)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene as substrates was meas...

Dietary absorption of lipophilic contaminants may be a significant route of exposure in aquatic organisms. Bile salts, lipids, and humic acids are important factors that may influence the intestinal absorption of a contaminant such as benzo[a]pyrene (BaP). We hypothesized that bile salts, monoglycerides, and free fatty acids would increase BaP intestinal absorption, while triglycerides, humic a...