Serum free fermentation procedures of cell cultures have got a wide application in production of biochemicals. But, cells cultured in serum free media in general are more sensitive to changes in culture condition, especially to nutrient limitation. There are no substances from serum which can support the cells when conditions are changing. In this study special attention is directed to amino ac...

BACKGROUND Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low rate of reaction. In the present study we have attempted to enhance the concentration of sugars in enzymatic hydrolysate of delignified Prosopi...

We consider the problem of active learning when the categories are represented as a tree with leaf nodes as outputs and internal nodes as clusters of the outputs at multiple granularity. Recent work has improved the traditional techniques by moving beyond ”flat” structure through incorporation of the label hierarchy into the uncertainty measure. However, these methods have two major limitations...

Microbial desalination cells (MDCs) have  great potential as a cost-effective and green technology for simultaneous water desalination, organic matter removal and energy production. The aim of this study was to compare the performance of a MDC under batch and continuous feeding conditions. Hence, power and current output, coulombic efficiency, electron harvest rate, desalination rate and COD re...

We present a constrained model-based optimization and predictive control framework to maximize the production efficiency of batch fermentations based on core idea manipulating adenosine triphosphate (ATP) wasting. In many bioprocesses, enforced ATP wasting —rerouting use towards an energetically possibly suboptimal path— allows increasing metabolic flux product, thereby enhancing product yields...

Two members of the AAA+ superfamily, ClpB and Hsp104, collaborate with Hsp70 and Hsp40 to rescue aggregated proteins. However, the mechanisms that elicit and underlie their protein-remodeling activities remain unclear. We report that for both Hsp104 and ClpB, mixtures of ATP and ATP-cS unexpectedly unleash activation, disaggregation and unfolding activities independent of cochaperones. Mutation...

Acetone-butanol-ethanol (ABE) fermentation process is a promising bioenergy option amid rising concerns over the environmental impact of fossil fuel usage. However, commercialization ABE has been marred by challenges low product yield and titer, thereby non-competitive economics. Here, we coupled cost competitive reducing agents with controlled feeding strategy to improve both titer yield. Redu...

Background and objective: Tetracyclines are the second most used group of antibiotics in the world. This type of antibiotic has a weak attraction in the body and enters wastewater through urine and feces. This study investigated the effectiveness of tetracycline removal from wastewater by air-cathode microbial fuel cells. Materials and methods: The current study was bench-scale experimental re...

We have analyzed the movement of single 22S dynein molecules from Tetrahymena cilia by using a nanometer measuring system equipped with optical tweezers. Statistical analysis proved that a single molecule of 22S dynein can move processively and develop force at low concentrations of ATP (<20 microM). The maximum force was approximately 4.7 pN, and the force-velocity curve was convex down. Durin...

Energy-efficient task scheduling is a fundamental issue in many application domains, such as energy conservation for mobile devices and the operation of green computing data centers. Modern processors support dynamic voltage and frequency scaling (DVFS) on a per-core basis, i.e., the CPU can adjust the voltage or frequency of each core. As a result, the core in a processor may have different co...