Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5–6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure ...

We investigate the anti-cancer stem cell (CSC) properties of two copper(II) complexes containing 4,7-diphenyl-1,10-phenanthroline and a S, N, X-Schiff base ligand, where X=O (1) or S (2). Complex 1 was previously reported by us, is first metal complex to induce cytotoxic immunogenic death breast CSCs. 2 structural analogue 1, phenolate moiety (within Schiff ligand) replaced thiophenolate group....

4-Hydroxyphenylpyruvate dioxygenase (I-hydroxyphenylpyruvate:oxygen oxidoreductase (hydroxylating, decarboxylating), EC 1.13.11.27) has been purified 800-fold in 25% yield from human liver by a procedure involving ammonium sulfate fractionation of an acetone powder extract and chromatography on hydroxylapatite, sulfopropyl-Sephadex C-50, and triethylaminoethyl cellulose. The preparation obtaine...

Three lisinopril-selective electrodes were developed with different techniques and in different polymeric matrices. Precipitation based technique with bathophenanthroline-ferrous as cationic exchanger in polyvinyl chloride (PVC) was used for the fabrication of sensor I (classical electrode) and sensor II (coated wire electrode). Hydroxypropyl ß cyclodextrin-based techniques were used for the fa...

The current study was designed to test the role of cytochrome P-450 in hydrogen peroxide-induced cytotoxicity, and to determine whether it may serve as a source of catalytic iron. Hydrogen peroxide led to iron release (as measured by iron/bathophenanthroline complex) from the microsomes prepared from LLC-PK1 cells. Cimetidine, which inhibits cytochrome P-450 by interacting with the heme iron, s...

The mechanism of low-affinity NGF receptor (LNGFR) truncation was investigated in cultured Schwann cells. Affinity labeling of Schwann cells with 125I-NGF or metabolic labeling with 35S-cysteine showed that truncated NGF receptor (NGF-Rt) was derived from the cell surface form of the receptor. Addition of full-length, exogenous NGF receptor (M(r) = 80 kDa) to Schwann cell membranes resulted in ...

Two Ru(II) polypyridyl complexes, Ru(DIP)2(bdt) (1) and [Ru(dqpCO2Me)(ptpy)](2+) (2) (DIP = 4,7-diphenyl-1,10-phenanthroline, bdt = 1,2-benzenedithiolate, dqpCO2Me = 4-methylcarboxy-2,6-di(quinolin-8-yl)pyridine), ptpy = 4'-phenyl-2,2':6',2″-terpyridine) have been investigated as photosensitizers (PSs) for photodynamic therapy (PDT). In our experimental settings, the phototoxicity and phototoxi...

Since its discovery in 2002, the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction—the most widely recognized example of click chemistry—has been rapidly embraced for applications in myriad fields. The attractiveness of this procedure (and its copper-free strained-alkyne variant) stems from the selective reactivity of azides and alkynes only with each other. Because of the fragile na...

The elucidation of flavonoid isomers is accomplished by electrospray ionization tandem mass spectrometry (ESI-MS/MS) via formation and collisional activated dissociation (CAD) of metal/flavonoid complexes containing an auxiliary ligand. Addition of a metal salt and a suitable neutral auxiliary ligand to flavonoids in solution results in the formation of [M(II) (flavonoid-H) ligand]+ complexes b...

The characterization and bioactivity of the dinuclear ruthenium(ii) complex [(Ru(DIP)2)2(tpphz)]4+ (DIP = 4,7-diphenyl-1,10-phenanthroline and tpphz = tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j]phenazine) is reported. This new complex is found to be luminescent in acetonitrile, where excitation into MLCT (metal-to-ligand charge-transfer) bands in the visible area of the spectrum (λex = 450...