Butyrylcholinesterase (BChE) has a major role in cocaine detoxication. The rate at which human BChE hydrolyzes cocaine is slow, with a kcat of 3.9 min(-1) and Km of 14 microM. Our goal was to improve cocaine hydrolase activity by mutating residues near the active site. The mutant A328Y had a kcat of 10.2 min(-1) and Km of 9 microM for a 4-fold improvement in catalytic efficiency (kcat/Km). Sinc...

The plant Cardaria draba (L.) Desv. subsp. (Brassicaceae), is traditionally used for various ailments in different cultures. We investigated solvent extracts such as methanol, ethanol, and water obtained from flowers, leaves, stems, roots of C. their chemical composition, well antioxidant enzyme inhibition activities acetylcholinesterase (AChE), butyrylcholinesterase (BChE), ?-glucosidase, ?-am...

Five mouse anti-human butyrylcholinesterase (BChE) monoclonal antibodies bind tightly to native human BChE with nanomolar dissociation constants. Pairing analysis in the Octet system identified the monoclonal antibodies that bind to overlapping and independent epitopes on human BChE. The nucleotide and amino acid sequences of 4 monoclonal antibodies are deposited in GenBank. Our goal was to det...