Metrics are a useful way to assess biocatalyst performance and, when compared techno-economic targets, can help set goals for further enzyme and bioprocess research development. Here, we outline some of the remaining challenges ensure wider acceptance this approach, both in industry academia.

The genus Rhodococcus has proved to be a promising option for the cleanup of polluted sites and application of a microbial biocatalyst. Rhodococcus sp. strain R04, isolated from oil-contaminated soil, can biodegrade polychlorinated biphenyls. Here we report the draft genome sequence of Rhodococcus sp. strain R04, which could be used to predict genes for xenobiotic biodegradation and provide imp...

A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platfor...

24 The genus Rhodococcus has proved to be a promising option for the 25 clean-up of polluted sites and application of a microbial biocatalyst. 26 Rhodococcous sp. strain R04, isolated from oil contaminated soil, could 27 biodegrade polychlorinated biphenyls. Here we report the draft genome 28 sequence of Rhodococcous sp. strain R04, which could be used to predict 29 genes for xenobiotic biodegr...

A two-step process is reported for the anomeric phosphorylation of galactose, using trehalose phosphorylase as biocatalyst. The monosaccharide enters this process as acceptor but can subsequently be released from the donor side, thanks to the non-reducing nature of the disaccharide intermediate. A key development was the creation of an optimized enzyme variant that displays a strict specificity...

The rootless duckweed Wolffia globosa, not explored toward electrogenicity till now, is investigated as a putative biocatalyst in Plant-based Biofuel Cells (P-BFC) for the electrical current generation and its basic metabolic changes during polarization are depicted. After short adaptation period, open-circuit voltage of P-BFC, utilizing W. globosa an anodic biocatalyst, reaches values 630 mV. ...

Prenylation plays an important role in diversifying the structure and function of secondary metabolites. Although several cyanobactin prenyltransferases have been characterized, their chemistries are mainly limited to modification electron-rich heteroatoms. Here we report a prenyltransferase, LimF, from Limnothrix sp. CACIAM 69d, geranylating electron-deficient C2 atom His imidazole. Interestin...